临床与实验病理学杂志Issue(11)：1232-1236,5.DOI:10.13315/j.cnki.cjcep.2014.11.008
子宫颈病变组织中HPV 16感染与TLR4介导的PI3 K/AKT信号通路相关蛋白表达的关系
Expression of Toll-like receptor 4 and protein of PI3 K/AKT signal pathway in cervical lesion and their relationship with HPV 16 infection
摘要
Abstract
Purpose To explore the expression of Toll-like receptor 4 (TLR4), PI3K, AKT and NF-κB in cervical lesions, and to in-vestigate their association with human papillomavirus ( HPV) 16 infection. Methods Immunohistochemical SP staining was performed to detect the expression of TLR4, PI3K, AKT, NF-κB in paraffin-embedded cervical tissue specimens from Uighur women with chroni-cal cervicitis, cervical intraepithelial neoplasia ( CIN) and cervical squamous cell carcinoma ( CSCC) . The HPV 16 DNA was detected by PCR. Results The positive expression rates of TLR4, PI3K, AKT, NF-κB in chronical cervicitis, CIN and cervical cancer were 32. 0%, 59. 4%, 77. 8%, 28. 0%, 56. 3%, 73. 0%, 24. 0%, 56. 3%, 79. 4%, and 8. 0%, 48. 4%, 81. 0%, respectively. The expression of them was higher in cervicitis than in CIN and cevical cancer ( P<0. 05 ) . The positive expression rates of HPV 16 in three groups were 8. 0%, 48. 4% and 81. 0% (P<0. 05). The expression of TLR4, PI3K, NF-κB and HPV 16 was related to cervi-cal cancer differentiation (P<0. 05). PI3K and AKT were significantly correlated with FIGOs’ stages (P<0. 05). NF-κB was corre-lated with lymph node metastasis. The expression of TLR4 was significantly associated with HPV 16 infection in CIN and CSCC ( r=0. 303, P=0. 015, r=0. 633, P=0. 000), and correlation with PI3K in CIN and CSCC (r=0. 254, P=0. 045, r=0. 386, P=0. 003). PI3K was associated with AKT only in CSCC (r=0. 298, P=0. 018). Conclusions The expression of TLR4 can be up-regulated by HPV 16 infection. High expression of PI3K/AKT signal pathway mediated by TLR4 may play important roles in the devel-opment and progression of CIN and CSCC, and HPV 16 infection may be a trigger factor affecting the molecular signal pathway.关键词
子宫颈肿瘤/鳞状细胞癌/Toll样受体4/PI3K/AKT信号通路/人乳头状瘤病毒16Key words
cervical neoplasm/squamous cell carcinoma/Toll-like receptors 4/PI3K/AKT signal pathway/HPV 16分类
医药卫生引用本文复制引用
石海燕,左艳丽,林齐睿,李巧稚,胡维维..子宫颈病变组织中HPV 16感染与TLR4介导的PI3 K/AKT信号通路相关蛋白表达的关系[J].临床与实验病理学杂志,2014,(11):1232-1236,5.基金项目
新疆自然科学青年基金(2012211B10) （2012211B10）
