临床与实验病理学杂志Issue(3)：306-308,3.DOI:10.13315/j.cnki.cjcep.2015.03.016
大鼠肝脏发育成熟及癌变时miR-100的表达
Expression changes of miR-100 in maturation and cancerous rat liver
嵇晓辉 1张晖 2杨金花 1朱武凌2
作者信息
- 1. 新乡医学院附属郑州人民医院病理科，郑州 450003
- 2. 新乡医学院病理学教研室，新乡 453003
- 折叠
摘要
Abstract
Purpose To explore the effect of miR-100 in rat liver development and the occurrence of liver cancer. Methods The liv-er tissues of neonate SD rats, adult SD rats and 2-AAF/PH rats were collected, then real-time fluorescence quantitative polymerase chain reaction ( RT-PCR) and FISH methods were used to detect the expression of miR-100 in the liver tissues. Results The expres-sion level of miR-100 in adult SD rats liver tissues was significantly higher than that in neonate SD rats liver tissues (P<0. 05). Com-pared with the adult rats, the expression level of miR-100 in 2-AAF/PH rats liver tissues was obviously decline (P<0. 05). Conclu-sion miR-100 can as a maker of liver mature, and its deletion maybe related to the tumor formation.关键词
肝肿瘤/miR-100/RT-PCR/FISHKey words
liver neoplasm/miR-100/RT-PCR/FISH分类
医药卫生引用本文复制引用
嵇晓辉,张晖,杨金花,朱武凌..大鼠肝脏发育成熟及癌变时miR-100的表达[J].临床与实验病理学杂志,2015,(3):306-308,3.
