临床与实验病理学杂志Issue(6)：607-610,4.DOI:10.13315/j.cnki.cjcep.2015.06.002
Annexin A3高表达对胃癌 MGC803细胞增殖及凋亡的作用
Effects of high Annexin A3 expression on apoptosis and proliferation of gastric cancer MGC-803 cells
摘要
Abstract
Purpose To eva1uate the effects of Annexin A3 on pro1iferation and apoptosis of gastric cancer ce11s. Methods The re-combinant p1asmid pYr-ads-4-Annexin A3 was constructed and ana1yzed by restriction ana1ysis and sequencing and was transfected into MGC803 ce11s. The stab1e transfectants were obtained after screening with G418. Western b1ot ana1ysis was used to examine the expres-sion of Annexin A3 before and after transfection. CCK8 assay,c1one assay and f1ow cytometry were used to study the effects of Annexin A3 on pro1iferation and apoptosis of MGC803 ce11s. Results The recombinant p1asmid pYr-ads-4-Annexin A3 was successfu11y con-structed. Western b1otting resu1ts indicated that the Annexin A3 expression was significant1y higher in ce11s transfected with pYr-ads-4-Annexin A3 compared with ce11s transfected with empty vectors and un-transfected ce11s( P<0. 05 ). CCK8 assay resu1ts showed the number of ce11s transfected with pYr-ads-4-Annexin A3 was significant1y higher than those transfected with empty vectors and un-trans-fected ce11s(P<0. 05). Moreover,the number of c1one in ce11s transfected with pYr-ads-4-Annexin A3 was significant1y higher than the other two groups(P<0. 05). Important1y,high Annexin A3 expression inhibited to apoptosis of MGC803 ce11s(P<0. 05). Con-clusion Annexin A3 expression p1ay important ro1es in tumorigenesis of gastric cancer. Annexin A3 cou1d promote the pro1iferation and inhibited apoptosis of gastric cancer ce11s and it might be a potentia1 target for gastric cancer treatment.关键词
胃肿瘤/Annexin A3/增殖/凋亡Key words
gastric neop1asms/Annexin A3/pro1iferation/apoptosis分类
医药卫生引用本文复制引用
张大勇,王连东,曾超..Annexin A3高表达对胃癌 MGC803细胞增殖及凋亡的作用[J].临床与实验病理学杂志,2015,(6):607-610,4.基金项目
国家自然科学青年基金 （）
