临床与实验病理学杂志Issue(7)：756-758,3.DOI:10.13315/j.cnki.cjcep.2015.07.009
比较实时荧光 PCR 法与 Sanger 测序法检测甲状腺乳头状癌BRAF基因突变
Comparison of real-time PCR method with Sanger sequencing for detection of BRAF muta-tion in papillary thyroid carcinoma
邱田 1黄文亭 1郭蕾 1鲁海珍 1凌云 1山灵 1李文斌 1吕宁 1应建明1
作者信息
- 1. 中国医学科学院北京协和医学院肿瘤医院病理科100021
- 折叠
摘要
Abstract
Purpose To investigate the positive rate and concordance rate of BRAF mutation in papillary thyroid carcinoma detected by real-time PCR method and Sanger sequencing. Methods 312 papillary thyroid carcinomas patients were enrolled in this study. Real-time PCR method and Sanger sequencing were performed to detect BRAF gene mutations. The frequency of BRAF mutation and the concordance of two methods were analyzed. Results BRAF mutation was detected in 65. 4% (204/312) and 63. 8% (199/312) of 312 papillary thyroid carcinoma samples by using real-time PCR method and Sanger sequencing, respectively. There was no significant correlation between BRAF gene mutations and patients’ gender. There was significant correlation between BRAF gene mutations and patients’ age. The overall concordance between real-time PCR method and Sanger sequencing for BRAF mutation detection was 98. 4%. Conclusion Real-time PCR method provides an effective method in BRAF gene mutation detection.关键词
甲状腺肿瘤/乳头状癌/BRAF基因突变/实时荧光PCR/Sanger测序Key words
thyroid neoplasm/papillary carcinoma/BRAF gene mutation/real-time PCR/Sanger sequencing分类
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邱田,黄文亭,郭蕾,鲁海珍,凌云,山灵,李文斌,吕宁,应建明..比较实时荧光 PCR 法与 Sanger 测序法检测甲状腺乳头状癌BRAF基因突变[J].临床与实验病理学杂志,2015,(7):756-758,3.
