中国临床保健杂志Issue(5)：519-522,4.DOI:10.3969/J.issn.1672-6790.2014.05.025
地黄低聚糖对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞凋亡的影响
Effect of rehmannia glutinosa oligosaccharides on hydrogen peroxide combined serum deprivation-induced apoptosis in adipose tissue-derived stem cells
摘要
Abstract
Objective To investigate the effect of Rehmannia glutinosa oligosaccharides (RGOs)on hydro-gen peroxide combined serum deprivation-induced apoptosis in adipose tissue-derived stem cells.Methods An apop-tosis model of miniswine adipose tissue-derived stem cells (ASCs)was established by hydrogen peroxide and serum deprivation (H2 O2 /SD)in vitro.RGOs (0.01 g/L,0.1 g/L,1 g/L,1 0 g/L)were pretreated for 1 2 hours and incuba-ted for 6 hours.Apoptosis of ASCs was determined by Annexin V-FITC /PI apoptosis kit,and the cell varibility and Caspase-3 activity was detected by CCK-8 assay and caspase-3 assay kit,respectively.Results RGOs significantly attenuated hydrogen peroxide combined serum deprivation-induced ASCs apoptosis,with a decreased apoptosis rate and caspase-3 activity,an increased cell varibility.Conclution RGOs has a protective effect on hydrogen peroxide-and serum deprivation-induced apoptosis of adipose tissue-derived stem cells.关键词
干细胞/细胞凋亡/地黄属/猪Key words
Stem Cell/Apoptosis/Hydrogen peroxide/Rehmannia/Swine分类
医药卫生引用本文复制引用
裴志勇,赵玉生,高伟,尹巧香..地黄低聚糖对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞凋亡的影响[J].中国临床保健杂志,2014,(5):519-522,4.基金项目
国家高科技研究发展计划 （）
