中国临床保健杂志Issue(3)：290-293,4.DOI:10.3969/J.issn.1672-6790.2015.03.021
miR-199在对顺铂敏感性不同的卵巢癌细胞株中的表达及其意义
The expression and significance of miR-199 in ovarian cancer cell lines
摘要
Abstract
Objective In this study,we aimed to detect the expression of miR -199 and searched for chemo-therapy sensitivity of indicators in ovarian cancer cell lines and.Methods Omics analysis had found that in ovarian cancer cell lines which had different sensitivities to cisplatin,the difference of expression of miR -199 is significant. Then used specificity methylation PCR and western blot to verify the expression level of P63 gene in ovarian cancer cell lines of the transient transfection miR199 mimic /antagomir.Results The expression level of miR-199 in ES-2 is much higher than in the expression of SKOV3 cells.The expression of p63 is negatively correlated with the expression of miR-199.Conclusions miR-199 and P63 may predict the sensitivity to cisplatin chemotherapy to some extent. miR-199 may effect the sensitivity to cisplatin chemotherapy of ovarian cancer by regulating its downstream target genes P63.关键词
卵巢肿瘤/抗药性,肿瘤/顺铂/紫杉酚/氟尿嘧啶Key words
Ovarian neoplasms/Drug resistance,neoplasm/Cisplatin/Paclitaxel/Fluorouracil分类
医药卫生引用本文复制引用
柳长青,徐汉杰,周颖,赵卫东..miR-199在对顺铂敏感性不同的卵巢癌细胞株中的表达及其意义[J].中国临床保健杂志,2015,(3):290-293,4.基金项目
国家自然科学基金（81272881）；安徽省科技攻关计划项目 （）
