辽宁农业科学Issue(6)：11-14,4.
番茄根结线虫病抗病基因 Mi 的 CAPS 标记及检测
CAPS Marker and Detection of Resistant Gene Mi inTomato
摘要
Abstract
In this paper,two resistant homozygous lines (Mi /Mi )and two homozygous susceptible lines (Mi /Mi )were amplified by specific primer.Both resistant and susceptible lines produced about 750 bp PCR frag-ment .After digestion with enzyme Taq I ,homozygous resistant genotypes and heterozygous resiatant genotypes could produce fragment about 570 bp,180 bp and 750 bp,570 bp,180 bp,respectively.While the susceptible genotypes still presented 750 bp fragment.Four hybrids were detected using this marker,and the results show that two hybrids are resistant heterozygous and two hybrids are susceptible.The marker also were used to de-tect F2 generation of 50 plants,the Separate ratio of resistant plants and susceptible plants is 3∶1 ,so genetic traits should be controlled by the quality characters.关键词
番茄根结线虫病/Mi/分子标记Key words
Tomato root-knot nematode Disease/Mi/Molecular marker分类
农业科技引用本文复制引用
吴媛媛,李海涛,吕书文,刘念,杨国栋,邹庆道,张子君..番茄根结线虫病抗病基因 Mi 的 CAPS 标记及检测[J].辽宁农业科学,2014,(6):11-14,4.基金项目
国家科技支撑计划项目 （）
