辽宁农业科学Issue(3)：76-78,3.DOI:10.3969/j.issn.1002-1728.2015.03.024
苹果 MD-ACO1启动子的克隆
MD-ACO1 Promoter Clonging
冉国栋1
作者信息
- 1. 青海省海东市乐都区现代农业示范园区管委会，青海 海东 810700
- 折叠
摘要
Abstract
Using the technic of CTAB,the whole DNA in apple(Malus pumila Mill.)cultivar ‘Rolay Gala’ was cloned.According to the apple ACO1 promoters gene that has published in the GenBank,constructing primer.Using PCR method Cloned the promoter fragments.A ACO1 promoter fragment which contains 953 bp and 975 bp was cloned.The sequence consistency rates of nucleotide was 98%,97%.We concluded that this gene fragment was MD-ACO1 promoter.关键词
苹果/ACO1/启动子/克隆Key words
Malus pumila Mill./ACO1/Promoter/Clonging分类
生物科学引用本文复制引用
冉国栋..苹果 MD-ACO1启动子的克隆[J].辽宁农业科学,2015,(3):76-78,3.
