皮肤性病诊疗学杂志Issue(5)：363-366,4.DOI:10.3969/j.issn.1674-8468.2014.05.003
利用MALDI-TOF-MS技术初步探讨不同中医证型的银屑病外周血T淋巴细胞膜蛋白
Proteomics Study of T Lymphocyte Membrane in Psoriasis Vulgar with Different TCM Syndrome Types by MALDI-TOF-MS Technology
吴玮 1范瑞强 1晏卉 1伍慧媚 2姚丹霓1
作者信息
- 1. 广东省中医院皮肤科，广东 广州 510120
- 2. 南京市妇幼保健院中医美容科，江苏 南京 210004
- 折叠
摘要
Abstract
Objective:To search proteins that might serve as T lymphocyte membrane biomark-ers for different TCM syndrome types of psoriasis vulgar by analyzing the proteomic components of T lymphocyte membrane from patients with psoriasis vulgar and normal controls .Methods:The anticoagulated blood from 30 patients with psoriasis vulgar including 10 blood-stasis syndrome、10 blood-heat syndrome and 10 blood-dryness syndrome and the anticoagulated blood of 10 normal people were collected to sort out T lymphocytes , and T lymphocyte membranes were extracted . The proteomics were detected by matrix-assisted laser desorption/ionization time of flight mass spectrometry ( MALDI-TOF-MS ) .Results:Four differentially expressed protein spots were ob-served between patients with psoriasis vulgar and normal controls .One differentially expressed pro-tein spot between blood-heat syndrome and blood-stasis syndrome and another one between blood-stasis syndrome and blood-dryness syndrome were successfully identified .No differentially ex-pressed protein spot was found between blood-heat syndrome and blood-dryness syndrome .Conclu-sion:There are some differences between T lymphocyte membrane proteins obtained from the pa-tients with psoriasis vulgar of different TCM syndrome types and normal controls , but more samples will be needed to confirm the results in future studies .关键词
寻常型银屑病/MALDI-TOF-MS技术/中医证型/淋巴细胞膜蛋白Key words
Psoriasis vulgar/MALDI-TOF-MS/TCM syndrome type/Lymphocyte membrane protein分类
医药卫生引用本文复制引用
吴玮,范瑞强,晏卉,伍慧媚,姚丹霓..利用MALDI-TOF-MS技术初步探讨不同中医证型的银屑病外周血T淋巴细胞膜蛋白[J].皮肤性病诊疗学杂志,2014,(5):363-366,4.
