河南科技大学学报（医学版）Issue(4)：245-246,250,3.
PIK3 CA基因在乳腺癌不同分子亚型中突变的研究
Study of PIK3CA Gene Mutation in Different Molecular Subtypes of Breast Cancer
袁鹏飞 1汪建光 1张丽柯 1靳高远 1刘德纯1
作者信息
- 1. 河南科技大学第一附属医院，河南洛阳 471003
- 折叠
摘要
Abstract
Objective To investigate correlation of PIK3 CA gene mutation in different molecular subtypes of breast cancer. Methods To select 200 cases of breast cancer tissue samples,The luminal subtype A, luminal subtype B,HER-2 over-expression subtype and besal-like subtype all the 50 cases.To extract DNA from samples of breast cancer, PCR amplification of exons 9 and 20 was done after determining the concentration and content by ultraviolet spectrophotometer, followed by sequence after the product was purified.Another 20 samples of adenosis of breast were chosen as controls. Results The frequency of PIK3CA mutations among 200 cases was 64 missense mutations (32%),26(40%)in exon 9,38(60%)in exon 20 were found.In molecular subtype of breast cancer:22(44%)in luminal subtype A ,16(32%)in luminal subtype B,20(40%)in HER-2 over-expression subtype,6(12%)in besal-like subtype,mutations distribution had statistical significance(P<0.05).No mutation was found in 20 cases of adenosis of breast. The mutation of PIK3 CA gene had no significant correlation with age,tumor size and lymph node status(P>0.05). Conclusion The mutations of PIK3CA gene with different molecular subtypes are different in breast cancer. However, there is no significant correlation between PIK3 CA gene mutations and the pathological features of breast carcinoma.关键词
PIK3 CA基因/突变/乳腺肿瘤Key words
PIK3 CA gene/mutation/breast neoplasms分类
医药卫生引用本文复制引用
袁鹏飞,汪建光,张丽柯,靳高远,刘德纯..PIK3 CA基因在乳腺癌不同分子亚型中突变的研究[J].河南科技大学学报（医学版）,2014,(4):245-246,250,3.
