林业科学Issue(6)：55-60,6.DOI:10.11707/j.1001-7488.20140607
马大杂种相思腋芽高效组培增殖体系
An Efficient Axillary Bud Multiplication System of Acacia mangium × A. auriculiformis
摘要
Abstract
To build an efficient axillary bud multiplication system of Acacia mangium × A. auriculiformis,the aseptic plantlets of A. mangium × A. auriculiformis were used to screen the optimal protocol for in vitro multiplication. The optimal multiplication medium and period,length of the sterile stems,subculture times,rooting and transplant survival rate were studied. The results showed that after being cultured 7 times on improved-MS+ 0. 5 mg·L -16-BA+0. 1 mg·L -1 NAA +0.04 g·L -1Ac(with combination of 3% sugar and 7 g·L -1 agar),which was the best multiplication medium screened from the trial test,the average proliferation times of the stem with one axillary bud were 2. 52,and the propagation coefficient of the plantlet was around 14. 99 after 35 days. Moreover,the plantlets grew well and there was no obvious variation of the multiplication ratio. The aseptic stem with 2 or 3 axillary buds was inoculated to 1/2MS +IBA1. 0 mg·L -1 +NAA0. 5 mg·L -1 ( with combination of 3% sugar and 7 g·L -1 agar) for rooting culture,the rooting rate was 96. 5% in 15 day and the transplant survival rate was 95. 0%.关键词
马大杂种相思/有效增殖倍数/单株繁殖系数/继代次数/腋芽增殖Key words
Acacia mangium × A. auriculiformis/efficient proliferation rate/propagation coefficient/subculture/axillary bud multiplication分类
农业科技引用本文复制引用
施琼,胡峰,黄烈健,詹妮..马大杂种相思腋芽高效组培增殖体系[J].林业科学,2014,(6):55-60,6.基金项目
国家“十二五”科技支撑计划项目(2012BAD01B0402)。 （2012BAD01B0402）
