林业科学Issue(6)：61-66,6.DOI:10.11707/j.1001-7488.20140608
台湾杉遗传多样性的ISSR分析
Genetic Diversity of Taiwania cryptomerioides Detected by ISSR Analysis
摘要
Abstract
The genetic diversity and genetic structure of four natural populations of Taiwania cryptomerioides were investigated using inter-simple sequence repeat ( ISSR) . Of the 100 primers screened,10 primers were selected and they generated 78 stable and distinct bands,among which 61 bands were polymorphic. The result of POPGENE indicated that T. cryptomerioides had a medium level of genetic variation at the species ( the percentage of polymorphic bands PPB =78. 21%,Nei’s gene diversity H=0. 249 4,Shannon’s information index I=0. 377 8) and population levels (PPB =52. 56%,H=0. 178 3,I=0. 267 7). There was to a certain level genetic differentiation among four populations (GST:0. 285 1; Shannon ’s genetic differentiation: 29. 14%; AMOVA genetic differentiation: 28. 31%) ,and most of the genetic differentiation occurred within populations. The UPGMA clustering and Mantel test showed that there was no significant correlation between the geographical and genetic distance(r = 0.043 3,P = 0.435 6). Based on these findings,we propose appropriate conservation strategies for this endangered species.关键词
台湾杉/ISSR/遗传多样性/遗传结构/保护策略Key words
Taiwania cryptomerioides/ISSR/genetic diversity/genetic structure/conservation strategy分类
农业科技引用本文复制引用
李江伟,杨琴军,刘秀群,季华,张卓文..台湾杉遗传多样性的ISSR分析[J].林业科学,2014,(6):61-66,6.基金项目
国家自然科学基金项目(30901130)。 （30901130）
