林业科学Issue(8)：102-107,6.DOI:10.11707/j.1001-7488.20140815
舞毒蛾LdOA1基因克隆分析及对3种杀虫剂胁迫的响应
Cloning and Analysis of LdOA1 in Lymantria dispar and Its Response to the Stress of Three Kinds of Insecticides
摘要
Abstract
The G protein coupled receptors ( GPCRs) ,known as transmembrane receptors,play an important role in drug research and development by activating G protein involved in inside signal transduction pathways. In present study, the full length cDNA of GPCR (namely LdOA1) was isolated from Lymantria dispar transcriptome. The open reading frame ( ORF) of LdOA1 was 453 bp encoding a protein of 150 amino acid residues with the molecular mass of 17. 54 kDa and theoretical pI of 9. 76. Phylogenetic analysis of GPCR proteins showed LdOA1 of L. dispar clustered into a group with Danaus plexippus. We further investigated the expression of LdOA1 in 3rd instar L. dispar larvae under deltamethrin, carbaryl and omethoate stresses using real-time PCR. The results showed that LdOA1 in L. dispar was obviously inhibited by deltamethrin,carbaryl and omethoate.关键词
舞毒蛾/LdOA1 基因/杀虫剂/基因表达Key words
Lymantria dispar/LdOA1/insecticide/gene expression分类
农业科技引用本文复制引用
曹传旺,孙丽丽,问荣荣,豆晓洁,王志英..舞毒蛾LdOA1基因克隆分析及对3种杀虫剂胁迫的响应[J].林业科学,2014,(8):102-107,6.基金项目
国家自然科学基金项目(31101676) （31101676）
东北林业大学青年拔尖人才支持计划(PYTT -1213-10) （PYTT -1213-10）
中央高校基本科研业务费专项基金(DL11BA02)。 （DL11BA02）
