林业科学Issue(8)：119-125,7.DOI:10.11707/j.1001-7488.20140817
梢枯病菌蛋白毒素对杂交竹线粒体结构和功能的影响
Effects of Protein Toxin from Arthrinium phaeospermum on Biophysical Characteristic and Respiration of Bambusa pervariabilis × Dendrocalamopsis grandis Mitochondria
摘要
Abstract
Effects of the protein toxin from Arthrinium phaeospermum on the membrane fluidity,surface electronic potential,swelling,aggregation and the contents of lipid peroxide MDA,Coenzyme Q 10 ( CoQ10 ) ,and respiration function of the mitochondria from shoots of different Bambusa pervariabilis × Dendrocalamopsis grandisvarieties were determined by the different detection techniques ( fluorescence polarization,neutral red dye,oxygen electrode,etc. ) . The results showed that the protein toxin caused the membrane fluidity weakening,surface electronic potential and aggregation decrease,swelling increase of mitochondria in four bamboo varieties. With the protein toxin treatment,the CoQ10 content decreased,the MDA content increase,the activities of cytochrome c oxidase ( CCO) and ATP decrease, respiratory control ratio ( RCR ) and oxidative phosphorylation ratio ( P/O ) significantly reduced. The above results demonstrated that the protein toxin damaged the mitochondrial membrane, increased the lipid peroxidation of mitochondrion,destroyed the membrane integrality,and inhibited the respiration. In term of all of the above indices,the effect of protein toxin on the resistant varieties (3# and 6#) was significantly weaker than that on the susceptible varieties (8# and 30#).关键词
蛋白毒素/线粒体/生物物理特性/呼吸作用Key words
protein toxin/mitochondrion/biophysical characteristic/respiration分类
农业科技引用本文复制引用
李姝江,谯天敏,韩珊,朱天辉,车冠男..梢枯病菌蛋白毒素对杂交竹线粒体结构和功能的影响[J].林业科学,2014,(8):119-125,7.基金项目
国家自然科学基金项目(31070578)。 （31070578）
