林业科学Issue(9)：82-88,7.DOI:10.11707/j.1001-7488.20140911
马尾松根际高效解磷真菌的筛选鉴定及其促生效应
Screening and Identification of Phosphate-Solubilizing Fungi of Pinus massoniana Rhizosphere and Its Application
摘要
Abstract
Phosphorus supply shortage in soil is one of reasons for decline of Pinus massoniana stands in China. Phosphate-solubilizing fungi ( PSF) are able to dissolve insoluble phosphorus,which is otherwise unavailable to plants, and transform it into soluble phosphorus. PSF can also promote plant growth to some extent. Since Masson pine stands are facing degradation of soil fertility in southern China,screening high-efficient PSF from rhizospheric soil of the forest and developing phosphate-solubilizing microbial fertilizers are an effective way to improve the soil nutrient and promote growth of the pine trees. To this end,in this work 45 phosphate-solubilizing fungi were isolated from 12 samples of P. massoniana rhizosphere in Nanjing Jiangsu and Huangshan Anhui of Eastern China,including 12 fungi that had strong capability to soluble phosphate. After a test of phosphate-solubilizing capability,two strains with strong phosphate-solubilizing capability were obtained: JP-NJ1 and JP-NJ4. Their solubilization of Ca3 ( PO4 ) 2 accounted for 1 051. 69 mg·L -1 and 872. 18 mg·L -1 , respectively. They belonged to Aspergillus awamori and Penicillium pinophilum, as identified by morphological observation and analysis of 18S rDNA sequences. The two efficient phosphate-solubilizing fungal strains were inoculated on P. massoniana seedlings and it was found that both the fungal suspension and their metabolite could promote growth of P. massoniana obviously and the root weight of P. massoniana was enhanced notably.关键词
马尾松/解磷真菌/解磷能力/18S rDNA/促生效应Key words
Pinus massoniana/phosphate-solubilizing fungi/phosphate-solubilizing capability/18S rDNA/effect of plant growth promoting分类
农业科技引用本文复制引用
魏伟,吴小芹,乔欢..马尾松根际高效解磷真菌的筛选鉴定及其促生效应[J].林业科学,2014,(9):82-88,7.基金项目
国家林业公益性行业科研专项(201004061) （201004061）
国家“十二五”科技支撑专题(2012BAD19B0703) （2012BAD19B0703）
江苏高校优势学科建设工程资助项目( PAPD)。 （ PAPD）
