林业科学Issue(9)：112-117,6.DOI:10.11707/j.1001-7488.20140915
楸树叶中抗氧化活性成分的分离和鉴定
Isolation and Identification of Bioactive Compounds with Antioxidant from Catalpa bungei
摘要
Abstract
Our previous study found that ethyl acetate extract phase of Catalpa bungei leaves had strong antioxidant activity. In order to further clarify its antioxidant activity,we have searched the components had strong antioxidant ability though silica gel column chromatography combined with DPPH free radical scavenging methods. The antioxidant components were isolated and purified by semi preparative liquid chromatography. With DPPH radical scavenging activity, reducing capacity and ABTS radical scavenging activity ability as investigation index to find the antioxidant components and the physicochemical properties and spectral data were to identify compounds. The results showed that the ethyl acetate extract phase of C. bungei leaves had two compounds: luteolin ( 1 ) and apigenin ( 2 ) . The antioxidant activity of compound 1 was stronger than BHT and compound 2. DPPH radical scavenging activity,reducing power and ABTS radical scavenging activity IC50 values of compound 1 were 23. 89,29. 77 and 43. 47 μg·mL -1 respectively. The antioxidant activity of compound 1 was stronger than compound 2 mainly because of its adjacent structure of two phenolic hydroxyl groups in the flavone compound nucleus of B ring. Luteolin and apigenin isolated from C. bungei leaves for the first time.关键词
楸树/抗氧化/木犀草素/芹菜素Key words
Catalpa bungei/antioxidant/luteolin/apigenin分类
农业科技引用本文复制引用
徐洪宇,张晓茹,王军辉,黄晓华,雷鸣,董娟娥,尉芹..楸树叶中抗氧化活性成分的分离和鉴定[J].林业科学,2014,(9):112-117,6.基金项目
国家自然科学基金项目(31170274) （31170274）
国家林业公益性项目科研专项(201204603) （201204603）
农业科技成果转化资金项目(2013GB24320611)。 （2013GB24320611）
