林业科学Issue(10)：74-79,6.DOI:10.11707/j.1001-7488.20141010
暗孢耳霉侵染竹梢凸唇斑蚜的生物学特征及毒力测定
Infection Features and Bioassay of Aphid-Obligate Conidiobolus obscurus on the Bamboo Aphid Takecallis taiwanus
摘要
Abstract
Bamboo forests are multi-function resources,which frequently suffer from bamboo aphid infestation. This study aimed to estimate the potential of the aphid-specific pathogen Conidiobolus obscurus on bamboo aphid biocontrol. Tthe fungal infection features on Takecallis taiwanus were observed by scanning electronic microscopy. Conidia adherent to the aphid cuticle could germinate rapidly to invade into host body. 24 h after inoculation,first cadavers appeared. Cystidia penetrated the cuticle in 6 h after host death,and followed by conidiophores,which sporulate for renewing next infection cycle. The result of multi-concentration bioassay showed that C. obscurus had high infectivity to T. taiwanus. After inoculated at high conidial concentration of ( 192. 6 ± 20. 3 ) conidia· mm - 2 ,92. 8% aphids died in 5 days. The bioassay data were well fitted to the time-concentration-mortality model, and the lowest value of the median lethal concentration was computed as 51. 6 conidia· mm - 2 on day 5. Meanwhile,the median lethal time was computed as 2. 3 days under the inoculation of 100 conidia· mm - 2 . All the results presented the promising potential of C. obscurus on bamboo aphid pest control.关键词
暗孢耳霉/竹梢凸唇斑蚜/生物测定/扫描电镜观察/生物防治Key words
Conidiobolus obscurus/Takecallis taiwanus/bioassay/scanning electronic microscopy/biocontrol分类
农业科技引用本文复制引用
王大伟,马良进,周湘..暗孢耳霉侵染竹梢凸唇斑蚜的生物学特征及毒力测定[J].林业科学,2014,(10):74-79,6.基金项目
国家自然科学基金项目(31300550) （31300550）
国际科学基金项目(IFS，C/5146－1)。 （IFS，C/5146－1）
