林业科学Issue(3)：155-162,8.DOI:10.11707/j.1001-7488.20150320
欧洲云杉扦插生根进程中内源激素和多酚类物质变化
Qualitative Analysis of Endogenesis Hormone and Polyphenol During Rooting of Cuttings in Norway Spruce (Picea abies)
摘要
Abstract
Objective]The high rooting efficiency of Norway spruce cuttings has important significance on promoting its superior clones breeding. The change of rooting characters,endogenous hormones and phenolics content in the rooting process of Norway spruce cuttings were studied in order to explore the effect of different endogenous hormone and polyphenols in cuttings on rooting and provide scientific theory basis for mechanism research of Norway spruce cutting propagation. [Method]In the present study,Norway spruce shoot cuttings from mixed clones of 5 -year-old trees were taken as the experimental material,whose callus formation and root development were investigated after cutting for 30,40, 50,55,60,65,70,75,and 70 d; as well as cuttings needles ( after 0,37,45,67,and 72 d) and cuttings base or basal callus ( after cutting for 45,67,and 72 d ) were analysised endogenous hormones and polyphenols content.[Result]The results showed that 55% shoot cuttings had formed callus in 30 d,which had finished in 50 d after planting. The period of 50 ~75 d after planting was fastigium of callus differentiation and adventitious root information,the rooting percentage was 90%,the average number of root was 10. 7. After which,the number of root remain the same,but the root was kept growing and the index of root effect was continue rising. The content of indoleacetic acid ( IAA ) ,zeatin /gibberellin ( ZT/GA3 ) reduced at 37 d after planting,28. 79% and 52. 35% respectively. whereas, the value of gibberellin ( GA3 ) ,ZT/IAA,GA3/IAA increased,55. 02%,32. 78% and 67. 97% respectively. During the period of callus formation (37 ~45 d) ,IAA and GA3 reduced 25. 75% and 40. 91% respectively;ZT/GA3 ,ZT/IAA and GA3/IAA increased,39. 32%,23. 75% and 20. 41% respectively. In the rooting induced period (45 ~67 d) ,the value of IAA, ZT/GA3 increased,IAA increased from 61. 7 to 79. 8 ng·100 g -1 . while,the value of GA3 ,ZT/IAA and GA3/IAA reduced 16. 27%,24. 86% and 35. 26% respectively. The level of ZT was about 80 ng·100 g -1 until 67 d after planting, which was decreased to 55. 6 ng·100 g -1 at 72 d. p-hydroxybenzoic acid ( PHBA ) level showed elevatory trend before 37 d,increased from 2. 23 to 7. 19 ng·100 g -1 ,which showed decrease trend after callus formation and reduced to 4. 16 ng·100 g -1 . Catechin ( CA ) and total phenolic acid ( TPA ) level both showed decrease trend in the rooting process. The change trend of endogenous hormones and polyphenol compounds in needles were the same as those in cutting base phloem. Endogenous hormones and polyphenols produced in young leaves and buds and transported to stem base and other parts. Therefore the content of endogenous hormones and polyphenol compounds in needles were much higher than that in cutting base phloem. Especailly for the level of IAA at 67 d,IAA level of needles (79. 8 ng·100 g -1 ) was 4. 58 ~7. 52 times higher than that of stem base (17. 4 ng·100 g -1).[Conclusion]In rooting process of shoot cuttings in Norway spruce,endogenous hormones and polyphenol jointly promoted callus differentiation and adventitious roots formation. The IAA level increased and GA3 ,ZT,and polyphenols decreased during the adventitious root formation period. Endogenous IAA was the main endogenous hormone for promoting adventitious root formation,endogenous GA3 ,ZT and polyphenol inhibited of adventitious root formation.关键词
欧洲云杉/嫩枝扦插/内源激素/酚类物质/生根进程Key words
Norway spruce/softwood cuttage/radication course/endogenesis hormone/polyphenol引用本文复制引用
欧阳芳群,付国赞,王军辉,马建伟,安三平,王美琴,李悦..欧洲云杉扦插生根进程中内源激素和多酚类物质变化[J].林业科学,2015,(3):155-162,8.基金项目
“十二五”国家科技支撑计划课题“北方针叶树种高世代育种技术研究与示范”(2012BAD01B01)。 （2012BAD01B01）
