林业科学Issue(4)：110-115,6.DOI:10.11707/j.1001-7488.20150414
杨树受溃疡病菌感染后转录因子的应答变化
Response of Transcription Factors of Populus tomentosa to Inoculation with Botryosphaeria dothidea
摘要
Abstract
Objective]This study aims at investigating the response of transcription-related genes of Populus tomentosa induced by Botryosphaeria dothidea infectation.[Method]The suppression subtractive hybridization ( SSH) technique was used to construct a cDNA library,and the bioinformatics were used to analyze the expression of transcription related gene of Populus tomentosa infected with Botryosphaeria dothidea. The bark mRNA was purified and transcribed into cDNA. A poplar-pathogen interaction cDNA library of inoculated poplar bark 72 h post-inoculation ( hpi) was constructed. Expressed sequence tags ( ESTs) were obtained,which were assembled into unisequences prior to their annotation. The unisequences related to transcription were selected,and were further analyzed by quantitative real-time PCR ( qRT-PCR) . The randomly selected 199 positive clones were sequenced,and compared with the sequences of NCBI gene pool,among which 172 EST sequences were found homologous. The homologous analysis for these EST was conducted in dbEST database retrieval,[Result]The result showed that 10 sequences were associated with transcription,and accounted for 5. 8% of the total sequences. They were divided into four categories,i. e. Zinc finger protein ( Cys / His-rich),containing the EAR transcriptional repressor; CONSTANS-like protein,Encoding a nuclear protein; ordinary transcription factor,regulating transcription and WRKY21 transcription factor,participating in the plant defense. Through further RT-qPCR analyses on three kinds of transcription factors,the result showed that the expression amount of Zinc finger protein ( Cys / His-rich ) after inoculation was 50 times of the non-inoculated;The WRKY21 expression amount was 0. 94 times of the non-inoculated;The CONSTANS-like protein expression amount was 2 times of the non-inoculated.[Conclusion]The expression of these transcription factor genes was significantly increased in Populus tomentosa infected by Botryosphaeria dothidea.关键词
葡萄座腔菌/毛白杨/抑制消减杂交/实时定量 PCR (qRT-PCR)/转录因子Key words
Botryosphaeria dothidea/Populus tomentosa/suppression subtractive hybridization/real time PCR ( qRT-PCR)/transcription factors分类
农业科技引用本文复制引用
张秀英,宋瑞清,张星耀..杨树受溃疡病菌感染后转录因子的应答变化[J].林业科学,2015,(4):110-115,6.基金项目
林业公益性行业科研专项“重大森林病虫灾害防控技术的关键理论基础”(201204501) （201204501）
中国林业科学研究院院部基金“林木病害和病原标本及病原体的收藏保存和评价”(CAFYBB2011005－4) （CAFYBB2011005－4）
科技部基础性工作专项“中国树木溃疡病原多样性及其生态地理分布和危害调查”(2009FY210100)。 （2009FY210100）
