泸州医学院学报Issue(1)：1-6,6.DOI:10.3969/j.issn.1000-2669.2015.01.001
游离脂肪酸激活肾系膜细胞NLRP3炎症小体信号及氧化应激机制的探讨
Investigation of the activation of NLRP3 inflammasome signaling and the mechanism of oxidative stress induced by free fatty acids in renal mesangial cells of rats
摘要
Abstract
Objective: To explore the expression of NLRP3 inflammasome induced by free fatty acids and the influence of oxidative stress in renal mesangial cells. Methods: Mesangial cells were randomly divided into the normal group(NC group), different concentrations of palmitic acid group(HPA group) and the NAC intervention in high palmitic group(HPA+NAC group). Each group was cultured for 6, 9 and 12 h,then detected the expressions of NLRP3, Caspase-1, IL-1β protein and mRNA by Western blotting and RT-PCR,and the influence on the protein and mRNA expression of each group induced by NAC was observed. Results: Compared with normal group, the protein and mRNA level of NLRP3, Caspase-1 and IL-1β were upregulated induced by different concentrations palmitic acid for various times,in a dose and time-dependent manner (P<0.05). Compared with palmitic acid group, the expressions of NLRP3, Caspase-1 and IL-1β were decreased after pretreatment palmitic acid growp with NAC(P< 0.05). Conclusion: Palmitic acid could induce the activation of NLRP3 inflammasome signaling in mesangial cells via reactive oxygen species and mediate inflammation in diabetic nephropathy.关键词
NLRP3/炎症小体/糖尿病肾病/棕榈酸/N-乙酰半胱氨酸Key words
NLRP3/Inflammasome/Diabetic nephropathy/Palmitic acid/N-acetyl-L-cysteine分类
医药卫生引用本文复制引用
刘霜,徐勇,吕诗诗,罗巧彦..游离脂肪酸激活肾系膜细胞NLRP3炎症小体信号及氧化应激机制的探讨[J].泸州医学院学报,2015,(1):1-6,6.基金项目
四川省国际合作项目 （）
