泸州医学院学报Issue(1)：11-14,4.DOI:10.3969/j.issn.1000-2669.2015.01.003
金钗石斛微粉细胞破壁率及其石斛碱含量测定
Determination of the rate of cell wall-breaking rate and content of Dendrobine in Dendrobium nobile micro-powder
摘要
Abstract
Objective: To determine the rate of cell wall-breaking and content of Dendrobine in Dendrobium nobile fines powder and micro-powder. Methods: The cell counting method was used to determine the cell breakage rate of Dendrobium nobile micro-powder, with parenchyma cells as evaluation index. The content of Dendrobine in Dendrobium nobileby was tested by gas chromatography. The weight of Dendrobium micro-powder sample was analyzed and the number of the complete parenchymal cells was counted. Results: The regression analysis showed that the weight of Dendrobium micro-poweder sample and number of complete parenchyral cells had a significant correlation. The cell wall-breaking rate was 100% after Dendrobium nobile was ultrafine grinded. And the content of fines powder and micro-powder were 0.42% and 0.35% respectively. Conclusion:This method is easy, quick and reliable, and can accurately evaluate the rate of cell wall-breaking and content of Dendrobine of Dendrobium nobile micro-powder .关键词
金钗石斛/微粉/细胞计数法/石斛碱/气相色谱法Key words
Dendrobium nobil/Micro-powder/Cell counting method/Dendrobine/Gas chromatography分类
医药卫生引用本文复制引用
罗颖,税丕先,傅秀娟,宁海燕,孙琴..金钗石斛微粉细胞破壁率及其石斛碱含量测定[J].泸州医学院学报,2015,(1):11-14,4.基金项目
四川省教育厅项目（14ZA0156）；泸州市科技局项目 （）
