泸州医学院学报Issue(2)：113-118,6.DOI:10.3969/j.issn.1000-2669.2015.02.001
2,2’,4,4’-四溴联苯醚染毒对大鼠学习记忆及海马NMDA受体亚基基因NR1和NR2B的影响
Effect of BDE-47 exposure on learning and the expression of genes memory and NR1 and NR2B of Hippocampal neurons in adult rat
摘要
Abstract
Objiective: To study the effect and mechanism of 2, 2', 4, 4'-tetrabromo-diphenyl ethers (BDE-47) exposure on spatial learning and memory abilities and the genes expression of NMDA-receptor subunit in adult rats. Methods: 80 healthy male SD rats were randomly divided into control group,vehicle group and three BDE-47-treated groups. The latter3 treated groups were treated by intragastric administration of 0.1 mg/kg, 0.5 mg/kg and 1.0 mg/kg BDE-47 once a day respectively. The rats in vechicle group received 1.0 mg/kg of peanut oil.The administration was lasted for 30 days. After experiment the capability of learning and memory of rats were measured by Morris water maze test. The expressions of both subunits of NMDA-receptor were evaluated by immunohistochemisty. Results: In BDE-47-treated groups the average escape latency was increased compared with control group (P< 0.05), and the freguency the animal crossed the original platform and the time spent in quadrant of the original platform were decreased compared with control group and dosage related (P< 0.05). The expression of NR1 and NR2B in CA1, CA3 and dentate gyrus areas of hippocampus were significantly decreased in the BDE-47 treated groups compared with the vehicle group and dosage related (P<0.05).And the expression mRNA of NR1 and NR2B showed statistically signifcant decrease in rats exposed to BDE-47. Conclusions:These findings show that the exposure to BDE-47 can induce behavioral alterations which may be due to the down-regulation of the genes expression of subunits of NMDA receptors.关键词
BDE-47/海马/NMDA受体/学习记忆Key words
2,2',4,4'-tetrabromo-diphenyl ethers/Hippocampus/N-methy-D-Aspartic acid/Learning and memory分类
医药卫生引用本文复制引用
且亚玲,汪春梅,阳静,汤艳..2,2’,4,4’-四溴联苯醚染毒对大鼠学习记忆及海马NMDA受体亚基基因NR1和NR2B的影响[J].泸州医学院学报,2015,(2):113-118,6.基金项目
四川省科技厅课题（2010JY0128）；四川省卫生厅项目基金 （）
