国际眼科杂志Issue(6)：1005-1008,4.DOI:10.3980/j.issn.1672-5123.2014.06.06
角膜不同保存方法与术后免疫排斥反应相关性的比较研究
Comparative study on the correlation of penetrating keratoplasty rejection with different cornea preservation methods
牛晓霞 1洪晶 2李云峰 1战露阳1
作者信息
- 1. 150076 中国黑龙江省哈尔滨市，哈尔滨爱尔眼科医院
- 2. 100191 中国北京市，北京大学第三医院眼科
- 折叠
摘要
Abstract
AIM: To understand the relation between the penetrating keratoplasty rejection and the methods of cornea preservation. <br> METHODS: The 30 Wistar rats as donator and 60 SD rats as receptor were used to establish the animal models of penetrating keratoplasty rejection. And 60 SD rats were randomly divided into 3 groups. Donor cornea of Wistar rats preserved in different methods were used separately in 3 groups. The penetrating keratoplasty rejection index ( RI ) , means survival time ( MST ) of corneal grafts and pathological changes in post -operation were analyzed. <br> RESULTS: The MST was ( 10. 4±1. 70 ) d in moist-chamber- preserved group (Ⅰ), ( 12. 9 ± 1. 81 ) d in medium-term-preserved group (II) and (16. 1±2. 57) d in cryopreserved group ( Ⅲ) . The MST in the cryopreserved group was evidently prolonged, showing a significant correlation compared with other two groups (P<0. 01). The sections with HE staining revealed that the severity of inflammation in Ⅲ group was reduced compared with that of Ⅰ, II group after 10d of keratoplasty. <br> CONCLUSION: The postoperative rejection of penetrating keratoplasty in rats is decreased and rejection time is delayed in cryopreserved cornea.关键词
保存方法/角膜移植排斥/大鼠Key words
preserving methods/penetrating keratoplasty rejection/rats引用本文复制引用
牛晓霞,洪晶,李云峰,战露阳..角膜不同保存方法与术后免疫排斥反应相关性的比较研究[J].国际眼科杂志,2014,(6):1005-1008,4.
