国际眼科杂志Issue(8)：1378-1381,4.DOI:10.3980/j.issn.1672-5123.2014.08.03
大豆黄酮玻璃体腔注射对大鼠视神经损伤的影响
Effect of Daidzein intravitreal injection on optic nerve injury in rats
摘要
Abstract
AIM:To discuss Daidzein intravitreal injection whether has protective and recovery effects on acute nerve damages. <br> METHODS:After the crush models of acute optic nerve were set up, 72 males SD rats were divided into 4 groups randomly as common group without surgery, FBS negative control group, Daidzein treatment group ( 10μmol/L, 100μmol/L, 1000μmol/L ) and positive control group using rats nerve growth factor ( mNGF, 100ng/mL ). Three days after interference, all experimental animals were executed. HE staining was used to evaluate morphologic change of the retina, immunohisochemical staining and western-blot tests for identifying and quantifying the distinct expression of Caspase-3 and GAP-43 among the groups. <br> RESULTS: Compared with the normal group and negative control group, retinal morphology of different concentrations of each Daidzein treatment group and positive control group was more complete, the expression of Caspase-3 protein was relatively lower, the expression of GAP-43 protein was relatively higher, the differences have statistically significance (P<0. 05).CONCLUSION: Daizein injection in the vitreous cavity has the capacity of protection and restoration in rat's acute nerve damages.关键词
大豆黄酮/视神经损伤/Caspase-3/GAP-43Key words
Daidzein/optic nerve damage/Caspase-3/GAP-43引用本文复制引用
唐中俊,黄振平,杨文静,邹勇象,蔡季平..大豆黄酮玻璃体腔注射对大鼠视神经损伤的影响[J].国际眼科杂志,2014,(8):1378-1381,4.基金项目
国家自然科学基金项目(No.81200719) （No.81200719）
