国际眼科杂志Issue(9)：1578-1583,6.DOI:10.3980/j.issn.1672-5123.2014.09.05
高糖对人视网膜色素上皮细胞神经钙黏连蛋白和纤维连接蛋白表达的影响
High glucose on the expression of N -cadherin and fibronectin in retinal pigment epithelium cells
毕文娇 1李睿姝 1侯定善 1范妍 1张晓梅1
作者信息
- 1. 150001 中国黑龙江省哈尔滨市，哈尔滨医科大学附属第一医院眼科
- 折叠
摘要
Abstract
To observe the expression of N - cadherin and fibronectin in retinal pigment epithelium ( RPE) cells in vitro under high glucose conditions, furthermore, to explore the effects of high glucose on epithelial -mesenchymal transition (EMT) in RPE cells. <br> ●METHODS: Human RPE (hRPE) cells were cultured in vitro. Containing a final concentration of 60mmol/ L glucose was used for high glucose treatment. The cells were divided into normal glucose group (5. 5mmol/ L, NG) and high glucose group (24, 48 and 72h) respectively. The expression of N - cadherin and fibronectin in hRPE cells were evaluated by immunofluorescence and real -time PCR. <br> ●RESULTS:RPE cells became disorganized and swollen over time under high glucose conditions, especially in 72h subgroup. lmmunohistochemical analysis revealed that the expression of N - cadherin in RPE cells under high glucose conditions was decreased compared with that in the control group, while the expression of fibronectin was increased. Real - time PCR results showed that the expression of N - cadherin mRNA in high glucose group was decreased at 24h compared with that in the control group, and declined markedly at 72h ( F = 12. 252, P =0. 000). There were no significant differences between the control group and the high glucose group at 24h, while the differences between the control group and the high glucose group (48 and 72h) were significant respectively (P < 0. 05 ). Meanwhile, the expression of fibronectin mRNA in RPE cells was increased in high glucose group at 24h, and reached the peak at 72h (F = 50. 543, P = 0. 000). There were no significant differences between the control group and the high glucose group at 24h. Compared with the control group, the expression of fibronectin mRNA in hRPE cells was increased significantly in high glucose group (48 and 72h) respectively (P= 0. 000, P= 0. 000). <br> ●CONCLUSlON: The expression of epithelium marker N-cadherin is down - regulated under high glucose conditions in hRPE cells in vitro. Meanwhile, the expression of mesenchymal maker fibronectin is induced and appeared to EMT changes. Results of this study will enrich our growing understanding in proliferative diabetic retinopathy and hopefully lead to novel insights for the pathogenesis and therapeutic treatments.关键词
人视网膜色素上皮细胞/高糖/上皮间质转化/神经钙黏连蛋白/纤维连接蛋白Key words
human retinal pigment epithelial cell/high glucose/epithelial - mesenchyal transition/N -cadherin/fibronectin引用本文复制引用
毕文娇,李睿姝,侯定善,范妍,张晓梅..高糖对人视网膜色素上皮细胞神经钙黏连蛋白和纤维连接蛋白表达的影响[J].国际眼科杂志,2014,(9):1578-1583,6.
