国际眼科杂志Issue(10)：1749-1754,6.DOI:10.3980/j.issn.1672-5123.2014.10.03
菊花总黄酮对去势导致干眼症雄兔泪腺细胞 Fas、FasL表达的影响
Effects of total flavonoid of chrysanthemum on Fas and Fasl expression in lacrimal gland cells of male castrated rabbits with dry eye
摘要
Abstract
AIM: To evaluate the effects of total flavonoid of chrysanthemum on the expressions of Fas and FasL in male rabbits with dry eye, and to investigate the therapeutic effects of the total flavonoid of chrysanthemum on dry eye. <br> METHODS: Totally 150 male Japanses white rabbits were divided into blank group ( group A ) , sham -operated group ( group B ) , model group ( group C ) , androgen control treatment group (group D), and total flavonoid of chrysanthemum treatment group ( group E ) . The dry eye model was established with orchiectomy on group C, D and E. Rabbits in group E were treated with total flavonoid of chrysanthemum. Rabbits in group D were treated with androgen intramuscular injection. Rabbits in the group A, group B, group C was treated with normal saline. All rabbits were detected with Schirmer's Ⅰ test and tear break-up time (BUT). Fas, FasL were checked on immunohistochemistry. <br> RESULTS:The Schirmer's I test values of group E was significantly higher than that of group C ( P<0. 01 ) and the BUT value of group E was significantly longer than that of group C ( P<0. 01 ). The quantity of positive expression of Fas in glandular tube cell and acinar epithelial celland apoptosis cells of group E after treatment at 1, 3, 5mo were significantly lower than that of group C, cell population of the positive expression of FasL was obviously higher than that of group C (P<0. 01). <br> CONCLUSION:The main component of chrysanthemum is flavonoid, which could significantly inhibit happening of dry eye in rabbit after androgen level lowered and lacrimal gland apoptosis and keep basic tears secretory volume and tear film stability.关键词
去势/干眼症/泪腺/细胞凋亡/菊花总黄酮Key words
castrate/dry eye/lacrimal gland/apoptosis/total flavonoid of chrysanthemum引用本文复制引用
姚小磊,彭清华,陈启雷,唐勇华,钟茜..菊花总黄酮对去势导致干眼症雄兔泪腺细胞 Fas、FasL表达的影响[J].国际眼科杂志,2014,(10):1749-1754,6.基金项目
国家自然科学基金地区基金项目(No.81260550) （No.81260550）
广西壮族自治区自然科学基金资助项目( No.2012GXNSFBA053092) （ No.2012GXNSFBA053092）
广西壮族自治区卫生厅中医药科技专项基金资助项目(No. GZPT1221)Local Foundation of National Natural Science Foundation (No.81260550) （No. GZPT1221）
Natural Science Foundation of Guangxi Zhuang Autonomous Region ( No.2012GXNSFBA053092) （ No.2012GXNSFBA053092）
Health Department of Pharmaceutical Technology Special Foundation, Guangxi Zhuang Autonomous Region (No. GZPT1221) （No. GZPT1221）
