国际眼科杂志Issue(10)：1755-1759,5.DOI:10.3980/j.issn.1672-5123.2014.10.04
Rhodopsin和 recoverin在 MNU诱导的光感受器损伤中的表达变化
Expression changes of Rhodopsin and recoverin in MNU-induced photoreceptor degeneration in rats
摘要
Abstract
AIM: To investigate the time - effect relationship between the expression of rhodopsin and recoverin and photoreceptor damage induced by N - nethl - N -nitrosourea ( MNU) . <br> METHODS: Thirty-six 7-week old Sprague-Dawley ( SD ) rats were intraperitoneally injected with MNU ( 60mg/kg ) and were put to death by dislocation of cervical vertebra 6, 12, 24h; 3, 7d after injection ( 6 per group) , respectively. As a control, six rats were injected with phosphate buffer saline (PBS) 5mL/kg and sacrificed on d3 after injection. The degree of photoreceptor apoptosis was detected by HE staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling ( TUNEL ) and transmission electron microscope ( TEM ) in the right eyes. The mRNA expressions of rhodopsin and recoverin were detected different time after injection by Western blot and immunohistochemical method in the left eyes. <br> RESULTS:The dissolution of photoreceptor nucleus and apoptosis body were first perceived at 12h by TEM; most of cells at outer nuclear layer were presented positive reaction. The apoptotic index reached peak ( 29. 7% ±2.3%) at 24h which was coincided with the observation of TEM. The results of immunohistochemistry displayed that rhodopsin and recoverin were on a declining curve with time extension. Furthermore, the results of Western blot indicated that rhodopsin had dramatic decline at 6h after injection (P<0. 05), and extremely significant difference comparing to control group after 12h ( P<0. 01 ); while recoverin dramatic declined at 12h, and extremely significant difference after 24h (P<0. 01). <br> CONCLUSION:60mg/kg MNU intraperitoneally injection one - time may specifically induce photoreceptor apoptosis, The mechanism of down - regulation of rhodopsin and recoverin may be related to the selected apoptosis of photoreceptors.关键词
N-甲基-N-亚硝基脲/视网膜/光感受器细胞/凋亡Key words
N - nethl - N - nitrosourea/retina/photoreceptor/apoptosis引用本文复制引用
金玮,邢怡桥,梅海峰,王文俊,杨安怀..Rhodopsin和 recoverin在 MNU诱导的光感受器损伤中的表达变化[J].国际眼科杂志,2014,(10):1755-1759,5.基金项目
教育部博士点基金(No.2013014112005) （No.2013014112005）
湖北省自然科学基金项目(No.2012FFB04401)Doctoral Fund of Education Ministry, China ( No.2013014112005) （No.2012FFB04401）
Natural Science Foundation of Hubei Province, China (No.2012FFB04401) （No.2012FFB04401）
