国际眼科杂志Issue(11)：1946-1949,4.DOI:10.3980/j.issn.1672-5123.2014.11.08
离焦诱导豚鼠近视眼视网膜超微结构及凋亡研究
Retinal apoptosis and ultrastructural features of defocus-induced myopia in guinea pigs
赵宏伟 1梁静南 2罗灵 1聂闯 3白凤华 1刘怡1
作者信息
- 1. 100101 中国北京市，解放军第 306 医院眼科
- 2. 100101 中国北京市，中国科学院微生物研究所
- 3. 100853 中国北京市，解放军第301医院
- 折叠
摘要
Abstract
AIM: To observe retinal ultrastructural features and retinal cell apoptosis of defocus induced myopia in guinea pigs.
<br> METHODS: Thirty guinea pigs ( 3 - wk old ) were received -10. 00D rigid contact lenses (RGP) on the right eye and without wearing glasses on the left eye. Diopter change with cycloplegic retinoscopy, ultrastructural features of the retina with transmission electron microscopy were observed, the apoptosis of retinal cell by TUNEL immunofluorescence staining after 3wk were detected.
<br> RESULTS: Eye diopter difference was 3. 28±0. 21D in the right eye and 1. 55±0. 23D in the left eye before and after induction. There was significantly statistical difference (P<0. 01);Retinal ultrastructural features were showed with transmission electron microscopy ( TEM ) the retinal disk membranes edema, some shedding, mitochondrial swelling and deformation, small amount of vacuolar changes in the inner and outer nuclear layer cells. Some characteristics of apoptosis were also be seen such as cell membrane irregular contractions, irregular nuclear chromatin aggregation. The incidence of retinal cell apoptosis was (2. 42±1. 24)% in the right eye and ( 0. 29±0.08 )% in the left eye after induction. There was significantly statistical difference (P<0. 01).CONCLUSION:Accompanied by abnormal apoptosis of retinal cells during the formation of defocus-induced myopia in guinea pigs.关键词
离焦诱导/豚鼠/近视眼/视网膜超微结构/凋亡Key words
defocus-induced/guinea pigs/myopia/retinal ultrastructural features/apoptosis引用本文复制引用
赵宏伟,梁静南,罗灵,聂闯,白凤华,刘怡..离焦诱导豚鼠近视眼视网膜超微结构及凋亡研究[J].国际眼科杂志,2014,(11):1946-1949,4.
