国际眼科杂志Issue(12)：2135-2138,4.DOI:10.3980/j.issn.1672-5123.2014.12.07
环孢素A 对兔晶状体上皮细胞增殖过程中磷酸肌醇-3激酶途径的影响
Effect of cyclosporine A on the Pl - 3 k pathway in proliferation of rat lens epithelial cells
摘要
Abstract
AlM:To investigate the effect of cyclosporine A ( CsA) on the phosphatidylinositol-3 kinase ( Pl-3k ) pathway during procession of proliferation in epithelial cells of rabbit lens, and provide treatment strategies for after cataract on the basis of experiment. <br> METHODS: Sixty eyes of 30 healthy white rabbits were operated by lens cortex removal in cataract surgery, and 30 right eyes were divided in treatment group and the other 30 eyes were divided in control group. From the first postoperative day, the control group eyes were dropped with normal saline 6 times each day, and the treatment group eyes were dropped with 1% CsA 6 times each day. Six rabbits were selected randomly and killed on the day before dropping and 1, 2wk, 1 and 2mo of postoperative day respectively. The lens of those killed rabbits were removed by surgery. The strategies of immunohistochemistry and mount in situ hybridization were used to detect the content of proliferating cell nuclear antigen ( PCNA) , gene of phosphate and tension homology deleted on chromsome ten ( PTEN) mRNA, Ser473-R, respectively. <br> RESULTS:The expression of PCNA and Ser473-R were both down-regulate after operation in treatment group and control group, and the PCNA levels were significantly lower among treatment group than those in control group on 1wk (0.690±0.035 vs 0.785±0.015, t=6.099, P<0.01) and 2wk (0. 571±0. 038 vs 0. 670±0. 037, t=4. 585, P<0. 01). ln addition, the levels of Ser473 - R were significantly lower among treatment group than those in control group on 1wk (0.374±0.031 vs 0.435±0.030, t=3.486, P=0.006) and 2wk (0. 220±0. 022 vs 0. 251±0. 020, t=2. 516, P=0. 031). However, the expression levels of PTEN mRNA were continually increased 1wk~1mo after operation, in which the expression levels of PTEN mRNA were significantly higher among treatment group than those in control group on 1wk (0. 302±0. 027 vs 0. 255±0. 038, t=2. 474, P=0. 033). <br> CONCLUSlON:1% CsA could inhibit the proliferation of epithelial cells in lens of rabbits with after cataract through preventing Pl-3k pathway.关键词
环孢素A/晶状体上皮细胞/后发性白内障/PTENKey words
cyclosporine A/lens epithelial cells/after cataract/gene of phosphate and tension homology deleted on chromsome ten引用本文复制引用
赵宁,张瑞君,钟一凡,刘磊,李佳..环孢素A 对兔晶状体上皮细胞增殖过程中磷酸肌醇-3激酶途径的影响[J].国际眼科杂志,2014,(12):2135-2138,4.基金项目
中国辽宁省自然科学基金项目(No.201102259)@@@@Natural Science Foundation of Liaoning Province, China (No.201102259) （No.201102259）
