国际眼科杂志Issue(12)：2139-2142,4.DOI:10.3980/j.issn.1672-5123.2014.12.08
缺氧诱导因子-1α及促红细胞生成素在大鼠角膜新生血管中的表达
Expression of hypoxia-inducible factor-1αand erythropoietin at corneal neovascularization in rats
王济民 1石蕊 2魏会玲 1马勇 2高丹1
作者信息
- 1. 715100 中国陕西省大荔县人民医院眼科
- 2. 710068 中国陕西省西安市，陕西省人民医院眼科
- 折叠
摘要
Abstract
AlM: To describe the expression of hypoxia-inducible factor-1α ( HlF-1α) and erythropoietin ( EPO ) in rats' corneal and evaluate its potential effect on corneal neovascularization ( CNV) growth. <br> METHODS:The young SD rats (3mo) was chosen and randomly divided into 2 groups, which were experimental group and normal control group. CNV model was established by alkali burn, and the length and area of CNV was observed everyday after operation by slit lamp. After that, the expression of HlF-1α and EPO was measured by SABC and RT-PCR methods at 1, 3, 5, 7, and 14d after alkali burn. The data was analyzed by SPSS 20. 0. <br> RESULTS:The area of CNV was increasing at 1, 3, 5, 7, and 14d after alkali burn, and the peak point appear at 7d. The growth speed was decreased after 14d. SABC method told us that no HlF-1αand very tiny amount EPO was detected at normal rats' corneal. The expression of the two factors increased at 1d after alkali burn in corneal epithelium and endoderm. The results of RT - PCR showed that a few amounts of HlF-1α and EPO mRNA were detected at normal group. The expression of the two factors was increased at 3d after alkali burn, and the peak value was found at 7d, however, it was decreased at 14d. Statistical difference was found at different time (P<0. 05). <br> CONCLUSlON: HlF- 1α and EPO is closely related to CNV.关键词
角膜新生血管/碱烧伤/缺氧诱导因子-1α/促红细胞生成素Key words
corneal neovascularization/alkali burn/hypoxia-inducible factor-1α/erythropoietin引用本文复制引用
王济民,石蕊,魏会玲,马勇,高丹..缺氧诱导因子-1α及促红细胞生成素在大鼠角膜新生血管中的表达[J].国际眼科杂志,2014,(12):2139-2142,4.
