国际眼科杂志Issue(3)：398-402,5.DOI:10.3980/j.issn.1672-5123.2015.3.05
PDR 患者外周血 CD4+CD25+T 细胞和相关基因 FOXP3的变化及临床意义
Changes and cIinicaI significance of CD4+CD25+T ceIIs and expression of FOXP3 mRNA in peripheraI bIood mononucIear ceII from patients with PDR
摘要
Abstract
· AlM:To investigate changes and significance of CD4+CD25+T cells and expression of FOXP3 mRNA in peripheral blood mononuclear cell ( PBMC ) from patients with proliferative diabetic retinopathy ( PDR ) and observe its clinical significance on pathogenesis of PDR. <br> · METHODS: Flow cytometry was used to analyze the proportion of CD4+CD25+T cells in PBMC with PDR and normal control, the expression of FOXP3, lL-17, CTLA-4 mRNA in patients with PDR, diabetes and normal control were detected by Q-PCR.The clinical data of glycosylated hemoglobin ( HbA1c), age, blood lipid, renal function and the degree of their relevance were analysed by Wilcoxon rank and inspection, Linear correlation analysis. ·RESULTS:CD4+T cells decreased, CD4+CD25+T with no significant difference, FOXP3 and CLAT-4 decreased in PDR, lL -17 increased in PDR patients. CD4+T cells associated with age, the level of CD4+CD25+T was positive correlation with the patient’s serum creatinine ( Cr ) , but had no significant with age, HbA1c, blood lipid, Cr and urea nitrogen ( BUN ). HbA1c, triglyceride ( TG ), cholesterol, low density lipoprotein ( LDL ) were higher than normal value in PDR patients. <br> · CONCLUSlON: CD4+CD25+T cells may be involved in the pathogenesis of PDR.ln addition, abnormal blood lipid indicate that PDR may be associate with blood lipid level.关键词
增殖性糖尿病视网膜病变/流式细胞术/CD4+CD25+T细胞/FOXP3Key words
proliferatiion diabetic retinopathy/flow cytometry/CD4+CD25+T cells/FOXP3引用本文复制引用
吴文一,唐罗生,陈慧慧,李芳..PDR 患者外周血 CD4+CD25+T 细胞和相关基因 FOXP3的变化及临床意义[J].国际眼科杂志,2015,(3):398-402,5.基金项目
国家自然科学基金 （）
