国际眼科杂志Issue(3)：407-410,4.DOI:10.3980/j.issn.1672-5123.2015.3.07
BMSCs 联合 ChABC 对视网膜变性大鼠光感受器细胞凋亡的影响
Bone mesenchymaI stem ceIIs and chondroitinaseABC on photoreceptor apoptosis in degenerated retina of rats
郑祥榕 1柳林 1高朋芬2
作者信息
- 1. 200433 中国上海市，第二军医大学附属长海医院眼科
- 2. 200127 中国上海市，上海交通大学医学院附属仁济医院眼科
- 折叠
摘要
Abstract
· AlM: To study the effect of bone mesenchymal stem cells ( BMSCs ) and chondroitinaseABC ( ChABC ) on photoreceptor apoptosis in the retina of sodium iodate-induced rats. <br> ·METHODS:Forty Sprague Dawley rats ( SD rats) were intraperitoneally injected with NalO3 (30g/L, 100mg/kg) to establish the retinal degeneration models ( postnatal 28d).These rats were devided into 4 groups.Group A was not injected, group B was injected with BMSCs, group C was injected with BMSCs and ChABC, and group D was injected with phosphate buffer saline ( PBS).After 28d, subretinal injection were applied. Hematoxyln - eosinstaining ( HE ) , tunel and immunohistochemistry were performed at 21d after subretinal injection. <br> · RESULTS: Photoreceptor number and photoreceptor apoptosis rate of B and C groups were more than those of A and D groups, and there was significant difference statistically ( P <0.05 ) . Photoreceptor number and photoreceptor apoptosis rate of group B were compared with those of group C, and there was no statistical significance between B and C groups ( P>0.05 ) .Glial fibrillary acidic protein ( GFAP) was expressed by BMSCs after intraocular injection. <br> · CONCLUSlON: BMSCs and ChABC injected into subretinal space may alleviate photoreceptor apoptosis so as to protect retinal photoreceptor cells in degenerated rats.关键词
骨髓间充质干细胞/硫酸软骨素酶/视网膜变性/凋亡Key words
bone mesenchymal stem cells/chondroitinaseABC/retinal degeneration/apoptosis引用本文复制引用
郑祥榕,柳林,高朋芬..BMSCs 联合 ChABC 对视网膜变性大鼠光感受器细胞凋亡的影响[J].国际眼科杂志,2015,(3):407-410,4.
