国际眼科杂志Issue(5)：759-763,5.DOI:10.3980/j.issn.1672-5123.2015.5.04
miR-181调控人晶状体上皮细胞凋亡的机制研究
Mechanism research of miR - 181 regulating human lens epithelial cell apoptosis
摘要
Abstract
•AlM: To investigate the expression of miR-181 in the lens tissue of cataract and the regulating mechanism of miR-181 on apoptosis of human lens epithelial cell. <br> •METHODS:Real time q-PCR was used to measure the expression of miR-181 in the anterior lens capsules of age - related cataract and human lens epithelial cell apoptosis model. miR- 181 mimic and inhibitor were transfected using Lipofectamine 2 000 to regulate the expression of miR-181, and then Real time q-PCR was used to verify transfection efficiency. Flow cytometry was used to detect the change of cell apoptosis rate. <br> • RESULTS: Compared with control group, the expression of miR-181 was significantly higher in both the anterior lens capsules of age-related cataract and human lens epithelial cell apoptosis model; the relative expression of miR-181 in lens epithelial cells transfected with miR-181 mimic was increased, whereas decreased in cells transfected with miR-181 inhibitor;the apoptosis rate of cells transfected with miR - 181 mimic was increased, while reduced in miR-181 inhibitor group. Each result was statistically significant (P<0. 01). <br> •CONCLUSlON:High expression of miR-181 is detected in anterior lens capsule of age-related cataract. miR-181 might play a certain role in the pathogenesis of cataract via promoting human lens epithelial cell apoptosis. miR-181 probably becomes a new approach for the nonoperative treatment of cataract, but the concrete mechanism still needs to be further studied.关键词
微小RNA/miR-181/细胞凋亡/白内障Key words
microRNA/miR - 181/cell apoptosis/cataract引用本文复制引用
秦宇,赵江月,罗文婷,李晶,刘佳,张劲松..miR-181调控人晶状体上皮细胞凋亡的机制研究[J].国际眼科杂志,2015,(5):759-763,5.基金项目
国家自然科学基金资助项目(No.81270988,81470617) （No.81270988,81470617）
辽宁省教育厅科学技术研究一般项目(No. L2014305)@@@@National Natural Science Foundation of China ( No.81270988,81470617) （No. L2014305）
Scientific Research Project of Education Department, Liaoning Province (No. L2014305) （No. L2014305）
