国际眼科杂志Issue(5)：764-766,3.DOI:10.3980/j.issn.1672-5123.2015.5.05
褪黑素对过氧化氢诱导晶状体上皮细胞氧化损伤的保护作用
Study of melatonin on the protective effect of hydrogen peroxide - induced oxidative damage in human lens epithelial cells
摘要
Abstract
•AlM: To investigate theprotective effect of melatonin against hydrogen peroxide ( H2 O2 )-induced oxidative damage to human lens epithelial cells. <br> •METHODS: Sub-culture human lens epithelial cells preprocessed with different concentrations of melatonin for 12h and then 100 μmol/L H2 O2 for 24h. The impact of melatonin on H2 O2-induced lens epithelial cell viability was detected by MTT assay, rate of apoptosis was detected by flow cytometry instrument and activity of apoptosis-related factors, Caspase-3 and Caspase-9, were detected by colorimetric method. <br> •RESULTS: MTT assay showed that melatonin had no effect on the activity of lens epithelial cells, and the drug can inhibit the decrease of H2 O2-induced cell activity, as well as flow cytometry showed that melatonin can inhibit H2 O2-induced apoptosis. ln addition, melatonin can also reduce H2 O2-induced Caspase-3 and Caspase-9 activity in lens epithelial cells, and their activity decreased with effect of melatonin along with extending time. <br> •CONCLUSlON: Melatonin can obviously inhibit H2 O2 -induced apoptosis of human lens epithelial cells, which provide reliable experimental basis for drug on treatment of cataract.关键词
褪黑素/晶状体上皮细胞/氧化损伤Key words
melatonin/human lens epithelial cells/oxidative damage引用本文复制引用
董天睿,刘平,倪锦红..褪黑素对过氧化氢诱导晶状体上皮细胞氧化损伤的保护作用[J].国际眼科杂志,2015,(5):764-766,3.基金项目
国家自然科学基金(No.30973275)@@@@National Natural Science Foundation of China (No.30973275) （No.30973275）
