中南医学科学杂志Issue(4)：339-342,4.
Kiss-1与结肠癌SW480细胞增殖的关系
Effect of Kiss-1 on the Proliferation of Colon Cancer Cell SW480
摘要
Abstract
Objective To use colon cancer cell SW480 as a model,to explore effects of overexpression of Kiss-1 on proliferation of colon cancer cells. Methods Using liposome transfection,Kiss-1/pEGFP-N1 recombinant plasmid was transfected into SW480 cells,colon cancer Kiss-1 cells were upregulated,stably expressed cells were selected by G418;the cells were divided into 3 groups:negative control group ( pEGFP-N1 transfection with empty vector ) , experimental group (with pEGFP-N1-Kiss1) and control group (without transfection). Western blot detection was used to detect the expression of Kiss-1 after transfection and CCK8 test was applied to analyze the inhibitory effect of Kiss-1 on the proliferation of SW480 cells. Results pEGFP-N1-Kiss-1 recombinant plasmid was successfully transfected into the colorectal cancer cell line SW480,Western blot detected low expression of Kiss-1 in the negative control group and blank control group,while Kiss-1 in experimental group was increased;CCK8 test showed significantly lower rate of proliferation of cells in the experimental group than the control group (P<0. 05). Conclusion Kiss-1 can inhibit the proliferation of colon cancer cells rate.关键词
结肠癌/Kiss-1/增殖Key words
colon cancer/Kiss-1/proliferation分类
医药卫生引用本文复制引用
钟华,吴雪艳,刘迪群..Kiss-1与结肠癌SW480细胞增殖的关系[J].中南医学科学杂志,2014,(4):339-342,4.基金项目
湖南省教育厅一般项目(11C1107) （11C1107）
