中南医学科学杂志Issue(4)：351-354,366,5.
PEITC通过抑制结肠癌细胞PI3 K/NF-κB活性而抑制MMP-9表达
Phenethyl Isothiocyanate Inhibits PI3 K/NF-κB to Down-regulate MMP-9 Expression in Human Colon Cancer Cells
摘要
Abstract
Objective To investigate the effect and molecular mechanism of PEITC on matrix metalloproteinase-9 ( MMP-9) expression in human colon cancer SW480 cells. Methods Human colon cancer SW480 cell line was cultured in vitro,and treated with 10,30,50μmol/L PEITC for 24h. Cell viability,cell migration and invasion was analyzed by MTT assay, scratch healing assay and transwell membrane assay,respectably. Expression of MMP-9 mRNA was analyzed by RT-PCR. Pro-tein expression and phosphorylation of Akt were detected by Western blot. Activity of NF-κB and MMP-9 were analyzed by lu-ciferase reporter assay. Results PEITC reduced SW480 cells invasion and migration without affecting the viability of cells, as demonstrated by scratch healing and transwell membrane assay. Furthermore, Western blot and RT-PCR showed PEITC markedly inhibited MMP-9 activity and expression at both protein and mRNA levels. PEITC attenuated Akt phosphorylation and activity of NF-κB. Moreover,LY294002,an inhibitor of PI3K,significantly inhibited NF-κB transcriptional activity and MMP-9 expression. In addition,different concentration of PEITC could inhibit the promotion activity of MMP-9. Conclusion PEITC as a potential anti-invasive agent by inhibiting MMP-9 is involved in PI3K/NF-κB pathways.关键词
异硫氰酸苯乙酯/SW480细胞/基质金属蛋白酶9/PI3K/Akt/核转录因子κBKey words
PEITC/SW480 cells/MMP-9/PI3K/AKT/NF-κB分类
医药卫生引用本文复制引用
魏付桥,申清香,刘昌化,罗康宁,谢文彪,胡军..PEITC通过抑制结肠癌细胞PI3 K/NF-κB活性而抑制MMP-9表达[J].中南医学科学杂志,2014,(4):351-354,366,5.基金项目
湖南省科技计划项目(2013SK3120) （2013SK3120）
