中南医学科学杂志Issue(4)：355-358,4.
哮喘豚鼠气道TGF-β1重塑及黄芪的干预研究
The Expression of TGF-β1 in the Airway Epithelial Cells of Asthmatic Guinea Pigs and the Effects of Huang Qi
摘要
Abstract
Objective To investigate the expression of TGF-β1 in the airway epithelial cell of asthmatic guinea pigs and study the effects of Huang Qi on airway remolding in asthma. Methods Forty adult male guinea pigs were randomly divided into control,asthmatic,arsenic Huang Qi and dexamethasone groups. HE staining and the image analysis were used to analyse the information of the airway remodeling. Immunohistochemistry was used to detect the expression of TGF-β1 in the lung tissue. Results Compared to control group,the model group showed that proliferation of airway smooth muscle,recon-struction of air passage,narrowing of bronchial lumen and swelling of airway epithelial cells were significantly milder after treatment with Huang Qi or dexamethasone. The airway wall and smooth muscle layer were significantly thicker in asthma group than that of control group,the number of smooth muscle cell nucleus increased significantly. The integrated optical den-sity ( IOD) value of TGF-β1 were statistically significantly higher than that in control which was derreased after treated with Huang Qi. Conclusions There was TGF-β1-overproduction in airway epithelial cells of asthmatic guinea-pigs,which may contribute to airway remolding in asthma. Astragus could decrease airway remolding by inhibiting the expression of TGF-β1.关键词
哮喘/黄芪/TGF-β1/气道重塑Key words
asthma/Huang Qi/TGF-β1/airwayremodeling分类
医药卫生引用本文复制引用
张满燕,熊花,陆煜,刘鑫..哮喘豚鼠气道TGF-β1重塑及黄芪的干预研究[J].中南医学科学杂志,2014,(4):355-358,4.基金项目
湖南省中医药管理局资助课题(201249) （201249）
