中南医学科学杂志Issue(1)：9-13,5.DOI:10.15972/j.cnki.43-1509/r.2015.01.003
PF4通过NF-κB上调巨噬细胞MMP-9表达
Platelet Factor 4 Up-regulates the Expression of Matrix Metalloproteinase-9 in Macrophages via NF-κB
摘要
Abstract
Objective To investigate whether PF4 modulates the MMP-9 expression of macrophages via Nuclear fac-tor kappa B(NF-κB). Methods THP-1 monocytes were differentiated into monocyte-derived macrophages by phorbol 12-myristate 13-acetate (PMA). Macrophages were incubated with PF4 (100 μg/L) in the absence or presence of NF-κB inhibitor pyrrolidine dithiocarbamate ( PDTC) ,the MMP-9 expression of macrophages was determined by Reverse-transcrip-tion polymerase chain reaction (RT-PCR). Macrophages were incubated with PF4 (25-200 μg/L) or vehicle (PBS),NF-κB content in cultured supernatant of macrophages was measured by ELISA assay. To evaluate the intracellular signal trans-duction pathways,macrophages were pretreated for 30min with the TLR4 blocker(monoclonal antibody HTA125,anti-TLR4) before the addition of PF4,NF-κB content was measured. Results Macrophages that were untreated showed a relatively low MMP-9 mRNA level;treatment with PF4 increased MMP-9 expression. However,the high levels of MMP-9 expression induced by PF4 were significantly attenuated in the presence of NF-κB inhibitor. PF4 increased concentration of NF-κB in a dose dependent manner,with the strongest increase at 100ng/mL. The increased the concentration of NF-κB induced by PF4 was significantly reduced when treated with TLR4 blocker. Conclusion PF4 may up-regulate MMP-9 expression in mac-rophages via NF-κB. TLR4 may be upstream signaling molecules of NF-κB in PF4-NF-κB signaling pathway.关键词
血小板因子4/基质金属蛋白酶9/巨噬细胞/核因子κB/动脉粥样硬化Key words
platelet factor 4/matrix metalloproteinase-9/macrophages/nuclear factor kappa B/atherosclerosis分类
医药卫生引用本文复制引用
赵战芝,何钒,唐雅玲,孙慧..PF4通过NF-κB上调巨噬细胞MMP-9表达[J].中南医学科学杂志,2015,(1):9-13,5.基金项目
国家自然科学基金(81100214),南华大学博士启动基金(2012XQD40) （81100214）
