中南医学科学杂志Issue(2)：132-136,5.DOI:10.15972/j.cnki.43-1509/r.2015.02.003
Caveolae在CGRP保护人脐静脉内皮细胞中的作用及机制
Role of Caveolae in the Protection of CGRP on the HUVECs Injury Induced by Oxidative
摘要
Abstract
Objective To study the role of caveolae on the protective effect of cacitonin gene-related peptide ( CGRP) on human umbilical vein endothelial cells ( HUVECs) injury induced by oxidative stress. Methods HUVECs were cultured in vitro,and treated with CGRP or / and hydrogen peroxide ( H2 O2 ) ,the distribution of cell cycles and prolif-eration of cells were observed by flow cytometry. The density and morphology of cells were observed by optical microscope,β-cyclodextrin (β-CD) was used to injured the structure of caveolae,expression of caveolin-1 was measured by Western blot. Rethods Compared with control group,treatment of CGRP increased proliferation index ( PI) and the number of S phase cells which were inhibited by H2 O2;Destruction of caveolae structure could increase the density of cells and change the cells shape;The expression of caveolin-1 was down-regulated by CGRP but up-regulated by H2 O2 ,respectively. Pretreat-ment with CGRP,the up-regulation of caveolin-1 induced by H2O2 was significantly reverted(P<0. 05). Pretreatment ofβ-cyclodextrin with cells,the expression of caveolin-1 induced by CGRP was furtherly decressed. Conclusions the de-struction of caveolae could increased the proliferative effect of CGRP on HUVECs induced by H2 O2 ,the mechanism involve in change of the expression of caveolin-1.关键词
过氧化氢/降钙素基因相关肽/caveolae/caveolin-1/内皮细胞Key words
H2 O2/CGRP/caveolin-1/caveolae/endothelial cells分类
医药卫生引用本文复制引用
朱怡,周孝钱,肖云彬,全海燕,骆竟妃,彭虹艳,秦旭平..Caveolae在CGRP保护人脐静脉内皮细胞中的作用及机制[J].中南医学科学杂志,2015,(2):132-136,5.基金项目
国家自然科学基金(30572192),湖南省青年科学基金课题(13JJ4121) （30572192）
