中南医学科学杂志2015，Vol.43Issue(3)：245-248,4.DOI:10.15972/j.cnki.43鄄1509/r.2015.03.002
组织芯片分析PRSS2蛋白在胃癌组织中的表达与意义
Significance and Expression of PRSS2 Proteins Analyzed by Tissue Array in Gastric Cancer Tissue
摘要
Abstract
Objective The significance and expression of PRSS2 protein was analyzed by tissue array in gastric cancer tissue and provide valuable data for finding diagnosis related protein of gastric cancer. Methods The 8 cases including normal gastric mucosa,cancer side tissue and gastric cancer were respectively collected. The expression of PRSS2 proteins was checked by Western-blotting. Then,the expression of PRSS2 protein was detected in gastric tissue arrays including normal gastric mucosa, cancer side tissue and gastric cancer by immunohistochemistry staining. The clinical pathology significance of expression of PRSS2 protein was analyzed in gastric cancer tissue. Results The expressions of PRSS2 protein was higher in gastric cancer tissue than that in normal gastric mucosa and cancer side tissue. It was higher in cancer side tissue than that in normal gastric mucosa. The expression positive rates of PRSS2 protein were 14. 29% (5/34),26. 92% (7/26) and 77. 78% (63/81) in normal gastric mucosa,cancer side tissue and gastric cancer tissue,respectively. The expressiong of PRSS2 was 60. 00% (3/5),70. 59% (12/17) and 81. 36% (48/59) in gastric well differentiated,middle differentiated and poorly differentiated adenocarcinoma,respec- tively. (P＜0. 01). There are negative relation between tissue differentiation level of gastric cancer and the expression positive rate of PRSS2(P＜0. 01). Conclusion The expression positive rate of PRSS2 protein was higher in gastric cancer tissue than that in normal gastric mucosa and cancer side tissue and higher in cancer side tissue than in normal gastric mucosa tissue. The expres-sion of PRSS2 protein was related with effluence and differentaiation level of gastric cancer tissue.关键词
胃癌/PRSS2蛋白/蛋白表达/分化程度Key words
Gastric cancer/PRSS2 protein/protein expression/differentaiation level分类
医药卫生引用本文复制引用
赵 强,舒友成,张志伟,肖娟,刘重元,伍石华,李臻,雷明生,杨代水,权里平..组织芯片分析PRSS2蛋白在胃癌组织中的表达与意义[J].中南医学科学杂志,2015,43(3):245-248,4.基金项目
湖南省自然科学衡阳联合基金( 12JJ9033 ) （ 12JJ9033 ）
湖南省自然科学基金( 12JJ3102、13JJ3079 ) （ 12JJ3102、13JJ3079 ）
湖南省科技厅项目( 2013SK3118、2014SK3081) （ 2013SK3118、2014SK3081）
湖南省高校创新平台基金(12K094、13K082、13K083) （12K094、13K082、13K083）
湖南省教育厅课题(11C1112、12C0340) （11C1112、12C0340）
衡阳市科技局项目(2013KJ12、2013KJ28) （2013KJ12、2013KJ28）
邵阳市科技局重点项目(2013SK13) （2013SK13）
湖南省卫生厅项目(B2013-048、B2014-163). （B2013-048、B2014-163）
