中南医学科学杂志2015，Vol.43Issue(3)：276-280,5.DOI:10.15972/j.cnki.43鄄1509/r.2015.03.010
miR-335在急性缺血性脑卒中表达下调及对钙调蛋白的调控作用
Negative Regulation of Calmodulin by miR-335 in Acute Ischemic Stroke
摘要
Abstract
Objective The miRNA dysfunction is suspected to be a contributing factor for many CNS pathologies in-cluding ischemic stroke. The present study investigates the expression of serum miR-335 in acute ischemic stroke ( AIS) , and explores the underlying mechanisms of miR-335 in acute ischemic stroke ( AIS) . Methods Blood samples were ob-tained from AIS patients (n=106) and healthy controls (n= 98). MiR-335 was measured by using a quantitative real-time PCR. Bioinformatics database assay was used to determine whether calmodulin gene (CALM1) was the direct target of miR-335. The miR-335 mimics and inhibitors were transfected into human umbilical vein epithelial cells ( HUVECs) with lipo-fectamine respectively. Calmodulin expression was determined by Western blot and reverse transcription-PCR ( RT-PCR) in the cultured HUVECs. Results Compared with the healthy control group,the expression of serum miR-335 was signifi-cantly lower in patients with AIS group (P＜0. 01). In addition,bioinformatics database assay show that the CALM1 present the binding sites,which was potentially targeted by miR-335. Finally,Transfection of the miR-335 mimic inhibited the ex-pression of CaM mRNA and protein in the HUVEC compared to the control group (P＜0. 01),while transfection of the miR-335 inhibitors increased the expression of CaM mRNA and protein (P＜0. 05). Conclusion MiR-335 down-regu-lation may increase the ischemic brain injury through up-regulating the expression of CaM.关键词
微小RNA-335/钙调蛋白/急性缺血性脑卒中Key words
microRNA-335/calmodulin/acute ischemic stroke分类
医药卫生引用本文复制引用
袁梅,汤永红,袁海军,周成芳..miR-335在急性缺血性脑卒中表达下调及对钙调蛋白的调控作用[J].中南医学科学杂志,2015,43(3):276-280,5.基金项目
衡阳市科技计划项目(2014KJ40). （2014KJ40）
