中南医学科学杂志2015，Vol.43Issue(3)：319-321,3.DOI:10.15972/j.cnki.43鄄1509/r.2015.03.024
HPLC联合UV法测定烟酸片中烟酸的含量
Determination of the Nicotinic Acid in Nicotinic Acid Tablets by HPLC Combined with UV
吴广 1曾赛丽2
作者信息
- 1. 南华大学附属第二医院药剂科,湖南 衡阳 421001
- 2. 南华大学附属第二医院呼吸内科
- 折叠
摘要
Abstract
Objective To establish an accurate and reliable HPLC/UV method for the quantitative determination of nicotinic acid in nicotinic acid tablets. Methods The chromatographic separation conditions employed for HPLV method were chosen using Aglient C18 column. 0. 02 mol/L potassium dihydrogen phosphate (Ph 6. 8)-acetonitrile (90:10) were used as the mobile phase pumped at a flow rate of 1. 0mL/min and a detection wavelength at 263 nm was used. UV method use potassium dihydrogen phosphate (Ph 6. 8)-acetonitrile (90:10) as solvent,and the detection wavelength were at 263 nm. The linearity,precision,accuracy,recovery rate and other content were tested to validate the method. Results The linear range of nictinic acid was 5. 0~100. 0 μg/mL. With UV method (r=0. 995 9),the average recovery is 99. 57%(RSD=1. 08%,n=6);with HPLC method (r=0. 999 8),the average recovery is 99. 62% (RSD=0. 74%,n=6).Conclusion The HPLC/UV method is simple,accurate,high-specificial and reproducible. This method can be used for the quality control nicotinic acid tablets.关键词
HPLC/UV/含量测定/质量控制/烟酸Key words
HPLC/UV/content determination/quality control/nicotinic acid分类
医药卫生引用本文复制引用
吴广,曾赛丽..HPLC联合UV法测定烟酸片中烟酸的含量[J].中南医学科学杂志,2015,43(3):319-321,3.
