中南医学科学杂志Issue(4)：369-373,5.DOI:10.15972/j.cnki.43-1509/r.2015.04.003
芹菜素激活c-Src/NADPH氧化酶/ROS通路诱导HO-1表达从而抑制小鼠巨噬细胞过度分泌细胞因子
Apigenin Induces HO-1 Expression by c-Src/NADPH Oxidase/ROS to Protect Against Excessive Cytokines Secretion in Murine Macrophages
摘要
Abstract
Objective To investigate the effect and the mechanism of Apigenin on lipopolysaccharides ( LPS)-induced cytokines production in murine macrophages. Methods The murine macrophage cell line RAW 264. 7 cells were cultured in vitro,and were treated with different concentration of Apigenin followed by LPS stimulation. Expression of heme oxygenase-1 (HO-1),phosphorylation of c-Src and p47phox,the subunit of NADPH oxidase were detected by Western blot;The intracellu-lar formation of reactive oxygen species ( ROS) was detected by using the fluorescent probe H2 DCFDA;Secretion of TNF-αand IL-6 were detected by ELISA. Results Western blot indicated that phosphorylated c-Src and p47phox were induced by apigenin after 30min of incubation. While 50μmol/L of PP1,an inhibitor of c-Src,could significantly inhibit p47phox phospho-rylation. In addition,Apigenin could also induce RAW264. 7 cells accumulation of ROS,and this effect could be abrogated by the NAPDH oxidase inhibitor,DPI. HO-1 could be induced by Apigenin,and pretreatment of PP1,DPI and the ROS scavenger N-acetyl-cysteine ( NAC) could block HO-1 production. Furthermore,apigenin could inhibit LPS-induced secretion of TNF-αand IL-6,and transfection of HO-1 siRNA could antagonize this action. Conclusion Apigenin can induce HO-1 expression by c-Src/NADPH oxidase/ROS to protect against excessive cytokines secretion in murine macrophages.关键词
芹菜素/血红素氧合酶-1/小鼠巨噬细胞Key words
Apigenin/Heme oxygenase-1/Murine macrophages分类
医药卫生引用本文复制引用
曾赛丽,吴广,谢莉,张秀峰,谭小武,何振华,游晓星,赵兰华,曾焱华..芹菜素激活c-Src/NADPH氧化酶/ROS通路诱导HO-1表达从而抑制小鼠巨噬细胞过度分泌细胞因子[J].中南医学科学杂志,2015,(4):369-373,5.基金项目
国家自然科学基金(31370207) （31370207）
