中南医学科学杂志Issue(4)：379-382,4.DOI:10.15972/j.cnki.43-1509/r.2015.04.005
铁皮石斛多糖对MPP+诱导的PC12细胞损伤的抑制作用
Inhibitory Effect of Polysaccharides from Candidum Dendrobium on the Injury Induced by 1-methyl-4-phenylpyridinium in PC12 Cells
摘要
Abstract
Objective To investigate the inhibitory effect of polysaccharides from candidum dendrobium on the inju-ry induced by 1-methyl-4-phenylpyridinium ( MPP+) in PC12 cells. Methods The PC12 cells were divided into con-trol,injury model and polysaccharides from candidum dendrobium (50,100,200 and 400μg/mL) group. The cells were in-cubated with 10μmol/L MPP+ for 24 h to induce the injury in injury model group. After the cells were pretreated with poly-saccharides from candidum dendrobium (50,100,200 and 400 μg/mL) for 2 hours,cells were incubated with 10 μmol/L MPP+ for 24 h in polysaccharides from candidum dendrobium group. The cells were incubated with the same volume of sa-line in control group. MTT assay was used to detect the survival rate of cells. The level of lactate dehydrogenase ( LDH) in the medium,the level of malondialdehyde ( MDA) in the cells and the activity of superoxide dismutase ( SOD) in the cells were measured. Results Compared with the control group,the survival rate of cells was singificantly decreased,the level of LDH in the medium and MDA in the cells were singificantly increased,the activity of SOD in the cells was singificantly decreased in the injury model group. Compared with the injury model group,the survival rate of cells was singificantly in-creased,the level of LDH in the medium and MDA in the cells were singificantly decreased,the activity of SOD in the cells was singificantly increased in the polysaccharides from candidum dendrobium (200 and 400 μg/mL) group. Conclusion Polysaccharides from candidum dendrobium inhibits the injury induced by MPP+ in PC12 cells,of which the mechanism may be related to the inhibition of oxidative stress induced by polysaccharides from candidum dendrobium.关键词
铁皮石斛/神经保护作用/氧化应激/PC12细胞Key words
candidum dendrobium/neuroprotective effect/oxidative stress/PC12 cells分类
医药卫生引用本文复制引用
董昕,廖慧颖,陆素青,李国术,符婉,潘思,张涛..铁皮石斛多糖对MPP+诱导的PC12细胞损伤的抑制作用[J].中南医学科学杂志,2015,(4):379-382,4.基金项目
衡阳市科技局课题(2012KJ22) （2012KJ22）
