中南医学科学杂志Issue(4)：383-387,5.DOI:10.15972/j.cnki.43-1509/r.2015.04.006
WIF-1、DKK基因启动子甲基化对COLO 320、HT-29细胞株β-catenin磷酸化的影响
Hypermethylation of WIF-1 and DKK Gene Family on the β-catenin Phosphorylation in Colorectal Adenocarcinoma Cell Lines COLO 320 and HT-29
摘要
Abstract
Objective To reveal the the effect of methylated inhibitory genes on Wnt/β-catenin pathway in colorec-tal adenocarcinoma cell lines. Methods With colorectal adenocarcinoma cell lines COLO 320,HT-29 and normal color-ectal cell line cCCD-18Co,methylation status of CpG island in promotor of WIF-1,DKK-1 and DKK-3 genes were deter-mined by methylation specific PCR (MSP),and mRNA levels of these genes were quantified by real-time RT-PCR,and phosphorylatedβ-catenin were semi-quantified by Western blot. Effect of DNA-demethylating agent 5-aza-2ˊ-deoxycytidine (5-aza-dC) treatment were also evaluated. Results DKK-1and DKK-3 mRNA decreased accompanied with hypermethy-lation status of the CpG islands in COLO 320,while DKK-3 and WIF-1 mRNA decreased accompanied with hypermethyla-tion in HT-29. Total β-catenin increased significantly while phosphorylated β-catenin declined in both cell lines. 5-aza-dC ameliorated these aberrant parameters partially. Conclusion The aberrant activation of Wnt/β-catenin pathway mediated by the hypermethylation of WIF-1 and DKKs genes may contribute to the oncogenesis of colorectal adenocarcinoma,while the methylation level of different Wnt inhibitory genes varied in colorectal cancer cell lines. Further research on this field will prompt the development of new therapies targeting the methylation process.关键词
Wnt/β-catenin信号通路/甲基化/结直肠癌/表观遗传学Key words
WNT/β-catenin signaling pathway/hypermethylation/colorectal adenocarcinoma/epigenetics分类
生物科学引用本文复制引用
周徽,胡光胜,曾斌,廖爱军..WIF-1、DKK基因启动子甲基化对COLO 320、HT-29细胞株β-catenin磷酸化的影响[J].中南医学科学杂志,2015,(4):383-387,5.基金项目
衡阳市科学技术局科技计划(编号:2011KJ64) （编号:2011KJ64）
