南京林业大学学报（自然科学版）Issue(6)：43-47,5.DOI:10.3969/j.issn.1000-2006.2014.06.009
乌桕种子成熟过程中种皮和胚乳超微结构观察
Ultrastructure of seed coat and endosperm in the development of Sapium sebiferum seed mature
摘要
Abstract
In order to know the mechanism of the dormancy of Sapium sebiferum seeds, we observed the changes of seed coat structure and endosperm nutrients of S. sebiferum seed during the process of maturity by using scanning electron mi⁃croscope ( SEM) and transmission electron microscope ( TEM) . The study showed that the hard and leathery seed coat composed four different parts:the cuticle, the palisade, the osteosclereids and the parenchymatous layer, arranged ac⁃cording to its position from the exterior to the interior. Compared to the other parts, the seed coat near radiculodium and the micropyle was thicker and the thickness increased as the seed matured. In addition, there was tallow both on the ex⁃terior and interior of the seed coat which was thought to be a barrier for exchanging water and gas with the environment. By the TEM, we found that at the early stage, the endosperm cells were characterized by the presence of amyloplasts, completeness of cell nucleus and other organelles, less black granules, and numerous vacuoles and lipid droplets. With the maturity of seed, the cell nucleus dwindled in size due to the dehydration, the starch grains transformed into sugar gradually. Maturation even led to the disappearance of vacuoles and lipid drops, and dissolution of organelles. While the black granules gathered in large quantities, indicating that the lipoprotein content increased, which would further intensi⁃fy the seed dormancy.关键词
乌桕/种子成熟/扫描电镜/透射电镜/种子休眠Key words
Sapium sebiferum/seed mature/SEM/TEM/seed dormancy分类
农业科技引用本文复制引用
廖卓毅,钱存梦,马秋月,徐晓梅,尹佟明,李淑娴..乌桕种子成熟过程中种皮和胚乳超微结构观察[J].南京林业大学学报（自然科学版）,2014,(6):43-47,5.基金项目
国家自然科学基金项目（31270711）；江苏省青蓝工程创新团队资助项目；江苏省研究生培养创新工程项目 （）
