南京林业大学学报（自然科学版）Issue(6)：48-54,7.DOI:10.3969/j.issn.1000-2006.2014.06.010
松材线虫与拟松材线虫种间杂交特性研究
Characteristics of hybridization between Bursaphelenchus xylophilus and B.mucronatus
摘要
Abstract
In this study, the hybridization was conducted between four Bursaphelenchus xylophilus strains selected from China, Japan, the United States and two B. mucronatus strains selected from China. The results showed that, hybridiza⁃tion could occur between some B. xylophilus strains and B. mucronatus strains, the success rate of negative crossing was two times that of positive crossing,but hybridization rate was extremely low, and the hybrid offspring was difficult to form a sustainable breeding population. The F1 hybrids larvae contained both Bx molecular markers and Bm molecular markers by using Bx/Bm quick molecular detection system. Inter⁃specific hybrids showed different gene types after subculturing 30 days, the offspring nematodes of BxAN5♀×Bm JNL1♂,BxAN5♂×BmAN5♀ and BxUSA♂×Bm JNL1♀ tended to become the populations which dominant species was Bx, as well as the offspring of BxJNL5♂×BmJNL1♀tended to be⁃come the population which dominant species was Bm. If intraspecific matings could happen, both B. mucronatus and B. xylophilus were inclined to have intraspecific mating priority. The adaptability of B. xylophilus in mating was better. There was little possibility of reproductive isolation between Bx and Bm which geographically close, and their inter⁃specific hy⁃brids were easily to produce. The reproductive isolation existed between B. mucronatus and B. xylophilus, and introgres⁃sion might happen but hybrids were difficult to form a sustainable reproductive offspring.关键词
松材线虫/拟松材线虫/生物学杂交/生殖隔离Key words
Bursaphelenchus xylophilus/B.mucronatus/hybrid/reproductive isolation分类
农业科技引用本文复制引用
应晨希,吴小芹..松材线虫与拟松材线虫种间杂交特性研究[J].南京林业大学学报（自然科学版）,2014,(6):48-54,7.基金项目
国家自然科学基金项目（31270683）；江苏省高校自然科学研究计划（11KJA22002）；江苏省普通高校研究生科研创新计划项目（ CXZZ110537）；江苏高校优势学科建设工程资助项目 （）
