南京林业大学学报（自然科学版）Issue(2)：47-51,5.DOI:10.3969/j.issn.1000-2006.2015.02.008
培养基种类及激素配方对青钱柳悬浮细胞次生代谢物质积累的影响
Influence of types of basic medium, hormone formulations on accumulation of secondary metabolites in Cyclocarya paliurus suspension cells
摘要
Abstract
Cyclocarya paliurus callus induced with its aseptic seedling were cultured in suspension culture. The types of basic medium, hormone formulations were studied to select the suitable Cyclocarya paliurus production of secondary metab⁃olites in plant cell suspension culture system. The results were shown as follows:The best medium of Cyclocarya paliurus suspension cell culture medium was MS, hormone formulations was 1.0 mg/L NAA+0.5 mg/L KT. The basic medium was MS which could increase the suspension cell dry weight and the production of secondary metabolites in the medium.B1(1�0 mg/L NAA+0.5 mg/L KT)hormone formulations was propitious to flavonoids accumulation in cell suspension.B3(1�0 mg/L IBA+0.5 mg/L TDZ) hormone formulations was propitious to polysaccharides accumulation in cell suspension.B2 (0�5 mg/L 2,4-D+0.3 mg/L NAA)hormone formulations was propitious to triterpenoid accumulation in cell suspension. Types of basic medium, hormone formulations on accumulation of secondary metabolites in Cyclocarya paliurus suspension cells were preliminarily selected. The results laid the foundation for the Cyclocarya paliurus suspension cell culture system.关键词
青钱柳/悬浮细胞/总黄酮/总三萜/总多糖Key words
Cyclocarya paliurus/suspension cells/total flavonoid/total triterpenoid/total polysaccharides分类
农业科技引用本文复制引用
阮氏钏,杨万霞,方升佐,曹燕妮..培养基种类及激素配方对青钱柳悬浮细胞次生代谢物质积累的影响[J].南京林业大学学报（自然科学版）,2015,(2):47-51,5.基金项目
国家自然科学基金项目（31270673）；江苏省研究生培养创新工程（CXLX12－0540）；江苏高校协同创新计划资助项目；江苏高校优势学科建设工程资助项目 （）
