南京林业大学学报（自然科学版）Issue(2)：57-62,6.DOI:10.3969/j.issn.1000-2006.2015.02.010
马占相思和大叶相思优树组培不定根诱导研究
Induction of adventitious roots during tissue culture of Acacia mangium and A. auriculiformis elite trees
摘要
Abstract
Ten years old Acacia mangium and A. auriculiformis elite individual trees were chosen as study materials in this experiment. Proliferated buds were obtained from stem explants being primarily cultured and proliferatively cultured. This study investigated factors related to rooting development of proliferated buds, such as type and concentration of aux⁃ins, type of basic medium, concentration of sucrose and active carbon. On the other hand, this experiment also studied the effects of proliferated buds induced by different medium on rooting rate. Results showed that the optimal rooting medi⁃um for A. mangium and A. auriculiformis was 1/2MS+IBA 0�5 mg/L+NAA 0�5 mg/L+sucrose 3% and 1/2MS+IBA 0�5 mg/L+NAA 0.25 mg/L+sucrose 3%, respectively. The rooting rate of both species could reach above 93%. Rooting rate of proliferated buds, after being cultured on proliferation medium with low concentration of cytokinin and active carbon, could reach above 90%, and remain stable with the increase of subculture time. Rooting seedlings grow strongly.关键词
马占相思/大叶相思/不定根诱导Key words
Acacia mangium/A. auriculiformis/adventitious roots induction分类
农业科技引用本文复制引用
胡峰,施琼,黄烈健..马占相思和大叶相思优树组培不定根诱导研究[J].南京林业大学学报（自然科学版）,2015,(2):57-62,6.基金项目
“十二五”国家科技支撑计划 （）
