南京林业大学学报（自然科学版）Issue(2)：111-115,5.DOI:10.3969/j.issn.1000-2006.2015.02.019
红柳制备低聚木糖和纤维低聚糖的研究
Research on xylo-oligosaccharides and cello-oligosaccharides preparation from Tamarix ramosissima
摘要
Abstract
Xylo⁃oligosaccharides and cello⁃oligosaccharides are benificial for health and they are usually prepared from crops, such as corn cob. In this work, sand⁃fixing pioneer plant, Tamarix ramosissima, was used as the raw material for preparing the xylo⁃oligosaccharides and cello⁃oligosaccharides. The 100 g dried Tamarix ramosissima was pretreated by steam explosion at 200℃ for 180 s, 8.74 g xylo⁃oligosaccharides and 49.8% xylo⁃oligosaccharides yield were obtained. When the steam⁃exploded Tamarix ramosissima was cooked in 25% alkali charge ( NaOH+Na2 S, 30% sulfidity) at a ra⁃tio of solid to liquid 1∶6, 160℃ for 90 min, the lignin removal and cellulose recovery were 96.2% and 82.8%, respec⁃tively. Three⁃stage enzymatic hydrolysis of steam exploded and sulfate pretreated Tamarix ramosissima by deffcientβ⁃glu⁃cosidase cellulase at dosage of 20μmol/( min·g) , substrate loading 5%, 50℃, pH 4.8 for 24 h, the yield of cello⁃oli⁃gosaccharides and the selectivity of cellulase to cello⁃oligosaccharides were 49.03% and 60.09%, respectively. The re⁃sults showed that the steam explosion was suitable for xylo⁃oligosaccharides preparation from Tamarix ramosissima, and three⁃stage enzymatic hydrolysis was beneficial for cello⁃oligosaccharides preparation from pretreated Tamarix ramosissima.关键词
红柳/低聚木糖/纤维低聚糖/三段水解Key words
Tamarix ramosissima/xylo-oligosaccharides/cello-oligosaccharides/three-stage hydrolysis分类
化学化工引用本文复制引用
周晓青,黄曹兴,李鑫,余世袁,勇强..红柳制备低聚木糖和纤维低聚糖的研究[J].南京林业大学学报（自然科学版）,2015,(2):111-115,5.基金项目
国家林业公益性行业科研专项项目（201404016）；江苏高校自然科学重大基础研究项目（11KJA220004）；江苏省六大人才高峰项目 （）
